DGP104- pSP64 Poly(A)700
(Plasmid
#70098)
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Purposean RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70098 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSP64
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2999
- Total vector size (bp) 3700
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Vector typein vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name700bp insert from B. subtilis
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SpeciesB. subtilis
- Promoter SP6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DGP104- pSP64 Poly(A)700 was a gift from David Gresham (Addgene plasmid # 70098 ; http://n2t.net/addgene:70098 ; RRID:Addgene_70098) -
For your References section:
Determination of in vivo RNA kinetics using RATE-seq. Neymotin B, Athanasiadou R, Gresham D. RNA. 2014 Oct;20(10):1645-52. doi: 10.1261/rna.045104.114. Epub 2014 Aug 26. 10.1261/rna.045104.114 PubMed 25161313