pNUT N6His mICA NG
(Plasmid #70162)

• Purpose: Expresses N-His tagged nonglycosylated mouse inhibitor of carbonic anhydrase
• Depositing Labs: Ross MacGillivray, Anne Mason
• Publication: Wang et al Biochem J. 2007 Aug 15;406(1):85-95.

Backbone

• Vector backbone: pNUT
• Backbone size w/o insert (bp): 7400
• Total vector size (bp): 9500
• Vector type: Mammalian Expression
• Selectable markers: Methotrexate

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mouse inhibitor of carbonic anhydrase
• Alt name: mICA
• Species: M. musculus (mouse)
• Insert Size (bp): 2136
• Mutation: Asn470Asp and Asn645Asp
• GenBank ID: NP08194
• Promoter: SV40
• Tag / Fusion Protein:
  • N-terminal signal peptide, 4 aa link, 6 His, Factor Xa cleavage site

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (not destroyed)
• 3′ cloning site: SmaI (not destroyed)
• 5′ sequencing primer: CCTTTGCGCCCGGACTCG
• 3′ sequencing primer: GTGGCAACTTCCAGGGCC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original clone was from the laboratory of Dr. Nancy Andrews then at Harvard Medical School. It was sent to the laboratory of Dr. R.T.A. MacGillivray at the University of British Columbia. It was inserted into the pNUT vector at UBC and sent to my lab for expression, purification and characterization.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry