pJK381
(Plasmid #71573)

• Purpose: Produces Thermoplasma acidophilum citrate synthase, Asp317>Gly mutant (TpCS-D317G)
• Depositing Lab: T. J. Kappock
• Publication: Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91. doi: 10.1021/bi9006447.

Backbone

• Vector backbone: pRec7
• Total vector size (bp): 3764
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: citrate synthase
• Alt name: gltA
• Alt name: TM
• Species: Thermoplasma acidophilum DSM 1728
• Mutation: changes aspartate-317 to glycine
• GenBank ID: TA_RS00855 P21553
• Promoter: recA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (destroyed during cloning)
• 3′ cloning site: AseI (destroyed during cloning)
• 5′ sequencing primer: lambda-PL promoter
• 3′ sequencing primer: custom

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Protein production is induced using nalidixic acid as described. (Kurz, L. C., Drysdale, G., Riley, M., Tomar, M. A., Chen, J., Russell, R. J. M., and Danson, M. J. ( 2000) Kinetics and mechanism of the citrate synthase from the thermophilic archaeon Thermoplasma acidophilum Biochemistry 39, 2283– 2296 DOI: 10.1021/bi991982r)

Also known as pJK383