pcDNA4/TO hTRPC5
(Plasmid
#72279)
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Purposemammalian expression of human TRPC5
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Depositing Lab
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Depositing OrganizationUniversity of Leeds
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72279 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA4/TO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5078
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTRPC5
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Alt nametransient receptor potential cation channel, subfamily C, member 5
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SpeciesH. sapiens (human)
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Entrez GeneTRPC5 (a.k.a. PPP1R159, TRP5)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA4/TO hTRPC5 was a gift from David Beech (Addgene plasmid # 72279) -
For your References section:
Human TRPC5 channel activated by a multiplicity of signals in a single cell. Zeng F, Xu SZ, Jackson PK, McHugh D, Kumar B, Fountain SJ, Beech DJ. J Physiol. 2004 Sep 15;559(Pt 3):739-50. Epub 2004 Jul 14. 10.1113/jphysiol.2004.065391 PubMed 15254149