Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pcDNA3.1-KozATG-dL5-2XG4S-actin
(Plasmid #73262)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73262 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5382
  • Total vector size (bp) 7338
  • Modifications to backbone
    The NdeI site was removed from the CMV promoter
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    No special instructions.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KozATG-dL5-2XG4S-actin
  • Alt name
    dL5-actin
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1950
  • Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L91S and NP138
  • Entrez Gene
    ACTB (a.k.a. BRWS1, PS1TP5BP1)
  • Promoter CMV
  • Tag / Fusion Protein
    • The FAP and actin are fused with 2 copies of a G4S linker

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMVF
  • 3′ sequencing primer BGHR
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.

http://pathways.mbic.cmu.edu/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-KozATG-dL5-2XG4S-actin was a gift from Marcel Bruchez (Addgene plasmid # 73262 ; http://n2t.net/addgene:73262 ; RRID:Addgene_73262)
  • For your References section:

    Localization microscopy using noncovalent fluorogen activation by genetically encoded fluorogen-activating proteins. Yan Q, Schwartz SL, Maji S, Huang F, Szent-Gyorgyi C, Lidke DS, Lidke KA, Bruchez MP. Chemphyschem. 2014 Mar 17;15(4):687-95. doi: 10.1002/cphc.201300757. Epub 2013 Nov 5. 10.1002/cphc.201300757 PubMed 24194371