pFastBacI/MDR3cysless
(Plasmid #74400)

• Purpose: expression of MDR3 in insect cells
• Depositing Lab: Robert Nakamoto
• Publication: Nakamoto lab plasmids (unpublished)

Backbone

• Vector backbone: pFastBacI
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4775
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MDR3
• Alt name: Pgp
• Species: M. musculus (mouse)
• Insert Size (bp): 3864
• Mutation: all Cys to Ala
• GenBank ID:
• Entrez Gene: Abcb1a (a.k.a. Abcb4, Evi32, Mdr1a, Mdr3, P-gp, Pgp, Pgy-3, Pgy3, mdr-3)
• Promoter: Polyhedrin
• Tag / Fusion Protein:
  • 10His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: Polyhedrin-F
• 3′ sequencing primer: pFastBac-Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Hassane Mchaourab, Vanderbilt University

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry