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PET14B BPSS2141
(Plasmid #78877)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 78877 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    PET14B
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4671
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BPSS2141
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

insert strain is Burkholderia pseudomallei

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PET14B BPSS2141 was a gift from Martino Bolognesi (Addgene plasmid # 78877)

  • For your References section:

    A structure-based strategy for epitope discovery in Burkholderia pseudomallei OppA antigen. Lassaux P, Peri C, Ferrer-Navarro M, Gourlay LJ, Gori A, Conchillo-Sole O, Rinchai D, Lertmemongkolchai G, Longhi R, Daura X, Colombo G, Bolognesi M. Structure. 2013 Jan 8;21(1):167-75. doi: 10.1016/j.str.2012.10.005. Epub 2012 Nov 15. 10.1016/j.str.2012.10.005 PubMed 23159127
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