pLVX-TetONE-Puro Rab5DN
(Plasmid #81027)

• Purpose: Rab5 dominant negative (S34N) expression
• Depositing Lab: Felicia Goodrum
• Depositing Organization: University of Arizona
  Located in the United States of America
• Publication: Goodrum lab plasmids (unpublished)

Backbone

• Vector backbone: pLVX-TetONE-Puro
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RAB5A
• Alt name: RAB5A, member RAS oncogene family
• Species: H. sapiens (human)
• Insert Size (bp): 1383
• Mutation: S34N dominant negative
• Entrez Gene: RAB5A (a.k.a. RAB5)
• Promoter: CMV, Lentiviral LTR
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: mCherry-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Subcloned from Addgene 35139 (p mCherry-N1 Rab5a DN)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry