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lpg1978_528-644_Mut
(Plasmid #83305)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83305 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET21a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    To produce protein, transform into Rosetta2(DE3)pLysS cells. induction: 30 degree @ 20 hours
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    lpg1978
  • Alt name
    Effector protein SetA
  • Alt name
    Subversion of eukaryotic traffic protein A
  • Species
    Legionella pneumophila subsp. pneumophila (strain Philadelphia 1)
  • Insert Size (bp)
    351
  • Mutation
    three mutations: K577A, K578A, and K580A
  • GenBank ID
    AAU28047.1
  • Promoter T7
  • Tag / Fusion Protein
    • Histidine tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lpg1978_528-644_Mut was a gift from SeWon Suh (Addgene plasmid # 83305 ; http://n2t.net/addgene:83305 ; RRID:Addgene_83305)

  • For your References section:

    Structural insights into SetA-mediated Rab1 glucosylation and PI3P-guided localization during early Legionella infection. Im HN, Lee Y, Song Y, Hahn H, Jeon H, Shin D, Lee S, Kim KH, Kim KT, Suh SW, Jang DM, Kim HS. Proc Natl Acad Sci U S A. 2026 Mar 31;123(13):e2535016123. doi: 10.1073/pnas.2535016123. Epub 2026 Mar 27. 10.1073/pnas.2535016123 PubMed 41894332
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