mCherry-PAK2 binding domain
(Plasmid
#83951)
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PurposeFLIM acceptor for mEGFP Rac1
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Depositing Lab
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Depositing OrganizationMax Planck Florida Institute
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83951 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 83951-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 83951-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonemCherry-C1
- Backbone size w/o insert (bp) 4645
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePak2
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SpeciesH. sapiens (human)
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Insert Size (bp)165
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Mutationaa60-113 only; S74A F84A
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GenBank IDNM_002577
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Entrez GenePAK2 (a.k.a. KNO2, PAK65, PAKgamma)
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Tags
/ Fusion Proteins
- mCherry (N terminal on backbone)
- mCherry (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 83951-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 83951-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-PAK2 binding domain was a gift from Ryohei Yasuda (Addgene plasmid # 83951 ; http://n2t.net/addgene:83951 ; RRID:Addgene_83951) -
For your References section:
Rho GTPase complementation underlies BDNF-dependent homo- and heterosynaptic plasticity. Hedrick NG, Harward SC, Hall CE, Murakoshi H, McNamara JO, Yasuda R. Nature. 2016 Sep 28;538(7623):104-108. doi: 10.1038/nature19784. 10.1038/nature19784 PubMed 27680697
Map uploaded by the depositor.
