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• Takuji Iwasato
• Luo et al
• pK168.AAV-EF1α-DIO-tTA-P2A-RF…
• Sequences

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Depositor Full Sequences (1)

Sequences from the depositing laboratory may be predicted or based on Sanger or NGS data. Addgene sequencing results may differ from the depositor-provided sequence.

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  Sequence > pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova) sequence 7115 bps CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTT TGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCC TGCGGCCGCACGCGTAAGCTTTGCAAAGATGGATAAAGTTTTAAACAGAGAGGAATCTTTGCAGCTAATG GACCTTCTAGGTCTTGAAAGGAGTGGGAATTGGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCC ACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAA ACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGC AGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGT TCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACTGGCTGCAGTA CGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCC CCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACC TTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCT TTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCC GCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCAC CGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTAT CGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCC GGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACAC AAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAG GCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATG GAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGG AATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCT TCCATTTCAGGTGTCGTGAGGTACCGGATCCTCTAGAGTCGACTCCGGAATAACTTCGTATAGGATACTT TATACGAAGTTATGCAGAATGGTAGCTGGATTGTAGCTGCTATTAGCAATATGAAACCTCTTAATAACTT CGTATAGCATACATTATACGAAGTTATGGCGCGCCTCATCTGTGCCCCAGTTTGCTAGGGAGGTCGCAGT ACTTGGCCACAGCCATCTCGTGCTGCTCGACGTAGGTCTCTTTGTCGGCCTCCTTGATTCTTTCCAGTCT GTGGTCCACGAAGTGGAAGCCGGGCATCTTGAGGTTCTTAGCGGGTTTCTTGGATCTGTATGTGGTCTTG AAGGAGCAGTGCAGGTAGCCCCCGCCCACGAGCTTCAGGGCCATCTGGCTGTGGCCTCTCAGGCCGCCGT CAGCGGGGTACAGCATCTCGGTGTTGGCCTCCCAGCCGCGTGTTTTCTTCTGCATCACAGGGCCGTTGGA TGGGAAGTTCACCCCGTTGATCTTGACGTTGTAGATGATGCAGCCGTTCTGGAAGCTGGTGTCCTGGGTA GCGGTCAGCACGCCCCCGTCTTCGTATGTGGTGATTCTCTCCCATGTGAAGCCCTCAGGGAAGGACTGCT TAAAGAAGTCGGGGATGCCCTGGGTGTGGTTGATGAAGGCTTTGCTGCCGTACATGAAGCTGGTAGCCAG GATGTCGAAGGCGAAGGGGAGAGGGCCGCCCTCGACCACCTTGATCTTCATGGTCTGGGTGCCCTCGTAG GGCTTGCCTTCGCCCTCGGATGTGCACTTGAAGTGGTGGTTGTTCACGGTGCCCTCCATGTACAGCTTCA TGTGCATGTTCTCCTTGATCAGCTCGCTCATAGGTCCAGGGTTCTCCTCCACGTCTCCAGCCTGCTTCAG CAGGCTGAAGTTAGTAGCTCCGCTTCCGGATCCCCCGGGGAGCATGTCAAGGTCAAAATCGTCAAGAGCG TCAGCAGGCAGCATATCAAGGTCAAAGTCGTCAAGGGCATCGGCTGGGAGCATGTCTAAGTCAAAATCGT CAAGGGCGTCGGCCGGCCCGCCGCTTTCGCACTTTAGCTGTTTCTCCAGGCCACATATGATTAGTTCCAG GCCGAAAAGGAAGGCAGGTTCGGCTCCCTGATGGTCGAACAGCTCAATTGCTTGTCTCAGAAGTGGGGGC ATAGAATCGGTGGTAGGTGTCTCTCTTTCCTCTTTTGCTACTTGATGCTCCTGATCCTCCAATACGCAGC CCAGTGTAAAGTGGCCCACGGCGGACAGAGCGTACAGTGCGTTCTCCAGGGAGAAGCCTTGCTGACACAG GAACGCGAGCTGATTTTCCAGGGTTTCGTACTGTTTCTCTGTTGGGCGGGTGCCGAGATGCACTTTAGCC CCGTCGCGATGTGAGAGGAGAGCACAGCGGAATGACTTGGCGTTGTTCCGCAGAAAGTCTTGCCATGACT CGCCTTCCAGGGGGCAGAAGTGGGTATGATGCCTGTCCAGCATCTCGATTGCCAGGGCATCGAGCAGGGC CCGCTTGTTCTTCACGTGCCAGTACAGGGTAGGCTGCTCAACTCCCAGCTTTTGAGCGAGTTTCCTTGTC GTCAGGCCTTCGATACCGACTTCATTGAGTAATTCCAGAGCAGAGTTTATGACTTTGCTCTTGTCCAGTC TAGACATGGTGGCGCTAGCATAACTTCGTATAAAGTATCCTATACGAAGTTATTTGCCTTAACCCAGAAA TTATCACTGTTATTCTTTAGAATGGTGCAAAGAATAACTTCGTATAATGTATGCTATACGAAGTTATGAA TTCGATATCAAGCTTATCGATAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTA ACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCG TATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTT GTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCA CCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTG CCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAATCA TCGTCCTTTCCTTGGCTGCTCGCCTATGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCC CTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCT TCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCATCGATACCGAGCGCTGCTC GAGAGATCTACGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCC AGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAAT ATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGG TCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCA AGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTT TTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGA TCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCT GATTTTGTAGGTAACCACGTGCGGACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTTGGCCACTCCCT CTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGC GGCCTCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGGGGCGCCTGATGCGGTATTTTCTCCTTACGCAT CTGTGCGGTATTTCACACCGCATACGTCAAAGCAACCATAGTACGCGCCCTGTAGCGGCGCATTAAGCGC GGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCT TTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAG GGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGG GCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTG TTCCAAACTGGAACAACACTCAACCCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTT CGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTT TACAATTTTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCG CCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCG TCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGT GATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGAC AATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGC CCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTG AGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATT ATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAA CCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTT TTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAAC TACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCT GCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGG CAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTC AGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTG AAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACC CCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAA AAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACT GGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGA ACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAA GTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGG GGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTAT GAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGG AGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTC TGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGT

Addgene-Verified Partial Sequences (2)

Addgene has verified portions of this plasmid. Results are shown below. Primers used for Sanger sequencing are indicated in the sequence header.

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  Sequence > EF1a-F 5'-TCAAGCCTCAGACAGTGGTTC-3' CaTtTCagGTGTCGTGAGGTACCGGATCCTCTAGAGTCGACTCCGGAATAACTTCGTATAGGATACTTTA TACGAAGTTATGCAGAATGGTAGCTGGATTGTAGCTGCTATTAGCAATATGAAACCTCTTAATAACTTCG TATAGCATACATTATACGAAGTTATGGCGCGCCTCATCTGTGCCCCAGTTTGCTAGGGAGGTCGCAGTAC TTGGCCACAGCCATCTCGTGCTGCTCGACGTAGGTCTCTTTGTCGGCCTCCTTGATTCTTTCCAGTCTGT GGTCCACGAAGTGGAAGCCGGGCATCTTGAGGTTCTTAGCGGGTTTCTTGGATCTGTATGTGGTCTTGAA GGAGCAGTGCAGGTAGCCCCCGCCCACGAGCTTCAGGGCCATCTGGCTGTGGCCTCTCAGGCCGCCGTCA GCGGGGTACAGCATCTCGGTGTTGGCCTCCCAGCCGCGTGTTTTCTTCTGCATCACAGGGCCGTTGGATG GGAAGTTCACCCCGTTGATCTTGACGTTGTAGATGATGCAGCCGTTCTGGAAGCTGGTGTCCTGGGTAGC GGTCAGCACGCCCCCGTCTTCGTATGTGGTGATTCTCTCCCATGTGAAGCCCTCAGGGAAGGACTGCTTA AAGAAGTCGGGGATGCCCTGGGTGTGGTTGATGAAGGCTTTGCTGCCGTACATGAAGCTGGTAGCCAGGA TGTCGAAGGCGAAGGGGAGAGGGCCGCCCTCGACCACCTTGATCTTCATGGTCTGGGTGCCCTCGTAGGG CTTGCCTTCGCCCTCGGATGTGCACTTGAAGTGGTGGTTGTTCACGGTGCCCTCCATGTACAGCTTCATG TGCATGTTCTCCTTGATCAGCTCGCTCATAGGTCCAGGGTTCTCCTCCACGTCTCCAGCCTGCTTCAGCA GGCTGAAGTTAGTAGCTCCGCTTCCGGATCCCCCGGGGAGCATGTCAAGGTCAAAATCGTCAAGAGCGTC AGCAGGCAGCATATCAAGGTCAAAGTCGTCAAGGGCATCGGCTGGGAgCATGTCTAAgTCAAAATCGTCA AGGGCGTCGGCCGGCCCGCCGCTTTcgcac
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  Sequence > WPRE-R 5'-CATAGCGTAAAAGGAGCAACA-3' CaGTCAaTCTTTCaCAAaTTTTGTaATCCAGAGGTTGATTATCGATAAGCTTGATATCGAATTCATAACt tCgTATAGCATACATTATACGAAGTTATTCTTTGCACCATTCTAAAGAATAACAGTGATAATTTCTGGGT TAAGGCAAATAACTTCGTATAGGATACTTTATACGAAGTTATGCTAGCGCCACCATGTCTAGACTGGACA AGAGCAAAGTCATAAACTCTGCTCTGGAATTACTCAATGAAGTCGGTATCGAAGGCCTGACGACAAGGAA ACTCGCTCAAAAGCTGGGAGTTGAGCAGCCTACCCTGTACTGGCACGTGAAGAACAAGCGGGCCCTGCTC GATGCCCTGGCAATCGAGATGCTGGACAGGCATCATACCCACTTCTGCCCCCTGGAAGGCGAGTCATGGC AAGACTTTCTGCGGAACAACGCCAAGTCATTCCGCTGTGCTCTCCTCTCACATCGCGACGGGGCTAAAGT GCATCTCGGCACCCGCCCAACAGAGAAACAGTACGAAACCCTGGAAAATCAGCTCGCGTTCCTGTGTCAG CAAGGCTTCTCCCTGGAGAACGCACTGTACGCTCTGTCCGCCGTGGGCCACTTTACACTGGGCTGCGTAT TGGAGGATCAGGAGCATCAAGTAGCAAAAGAGGAAAGAGAGACACCTACCACCGATTCTATGCCCCCACT TCTGAGACAAGCAATTGAGCTGTTCGACCATCAGGGAGCCGAACCTGCCTTCCTTTTCGGCCTGGAACTA ATCATATGTGGCCTGGAGAAACAGCTAAAGTGCGAAAGCGGCGGGCCGGCCGACGCCCTTGACGATTTTG ACTTAGACATGCTCCCAGCCGATGCCCTTGACGACTTTGACCTTGATATGCTGCCTGCTGACGCTCTTGA CGATTTtGACCTTGACATGCTCCCCGGGGGATCCGGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAG GCTGGAgACGTGGAGGAGAACCCTGGACCTATGAGCGAGCTGATCAAGGAGAACATGCACATGAAGCTGT ACATGGAgggc