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MSCV(pBabemcs)-human CSF-1(256)-IRES-GFP
(Plasmid #86795)


Item Catalog # Description Quantity Price (USD)
Plasmid 86795 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6573
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    colony stimulating factor 1
  • Alt name
    macrophage colony stimulating factor 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    256, membrane bound form
  • Entrez Gene
    CSF1 (a.k.a. CSF-1, MCSF, PG-M-CSF)
  • Tag / Fusion Protein
    • IRES-GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pLXSN5; pBABE5
  • 3′ sequencing primer pCDH-rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The mcs is from the pBabe vector that was inserted into the MSCV vector to provide more cloning sites, and was developed by Dr Den Besten. The human CSF-1 cDNAs were cloned by the Cetus Company.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV(pBabemcs)-human CSF-1(256)-IRES-GFP was a gift from Martine Roussel & Charles Sherr (Addgene plasmid # 86795 ; ; RRID:Addgene_86795)
  • For your References section:

    Synthesis, post-translational processing, and autocrine transforming activity of a carboxylterminal truncated form of colony stimulating factor-1. Heard JM, Roussel MF, Rettenmier CW, Sherr CJ. Oncogene Res. 1987 Sep-Oct;1(4):423-40. PubMed 2966924