pCDNA-FRT-TO-Flag-TZAP
(Plasmid
#87177)
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PurposeInducible stable expression of Flag-TZAP using the FLP-In T-Rex system
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Depositing Lab
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Depositing OrganizationScripps California (The Scripps Research Institute)
Located in the United States of America -
Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87177 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCDNA5-FRT-TO
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTZAP
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SpeciesH. sapiens (human)
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Entrez GeneZBTB48 (a.k.a. HKR3, TZAP, ZNF855, pp9964)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA-FRT-TO-Flag-TZAP was a gift from Eros Lazzerini Denchi (Addgene plasmid # 87177) -
For your References section:
TZAP: A telomere-associated protein involved in telomere length control. Li JS, Miralles Fuste J, Simavorian T, Bartocci C, Tsai J, Karlseder J, Lazzerini Denchi E. Science. 2017 Jan 12. pii: aah6752. doi: 10.1126/science.aah6752. 10.1126/science.aah6752 PubMed 28082411