Skip to main content
Addgene
Open Menu

pMSCV-p53DD-IRES-EYFP
(Plasmid #89551)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89551 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    MSCV
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TP53
  • Alt name
    p53
  • Species
    H. sapiens (human)
  • Mutation
    truncated cDNA encodes truncated protein (aa 300-393) with dominant negative function, deletion mutant is called "DD"
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter MSCV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A dominant negative p53 construct (p53DD) (Shaulian et al., 1992), a gift from M. Oren, was subcloned using EcoRI into pMSCV-IRES-EYFP, generating pMSCV-p53DD-IRES-EYFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-p53DD-IRES-EYFP was a gift from Alf Grandien (Addgene plasmid # 89551)
Related items: