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pre-Ubc4 pUC19
(Plasmid #90309)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90309 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UBC4
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    UBC4 (a.k.a. YBR082C)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7 Fwd
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pre-Ubc4 pUC19 was a gift from Christine Guthrie (Addgene plasmid # 90309)

  • For your References section:

    Preparation of fluorescent pre-mRNA substrates for an smFRET study of pre-mRNA splicing in yeast. Abelson J, Hadjivassiliou H, Guthrie C. Methods Enzymol. 2010;472:31-40. doi: 10.1016/S0076-6879(10)72017-6. 10.1016/S0076-6879(10)72017-6 PubMed 20580958
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