YSBE 557 : pNAT_GFP
(Plasmid
#92085)
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PurposePlasmid for tagging proteins in Cryptococcus neoformans; GFP tag, NAT selection
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Depositing Lab
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Depositing OrganizationYonsei University
Located in Republic of Korea -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 92085 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 92085-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 92085-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTOP-V2
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Backbone manufacturerEnzynomics, Korea
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Vector typetagging proteins in Cryptococcus neoforman
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Selectable markersNourseothricin (NAT)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGFP
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 92085-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 92085-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YSBE 557 : pNAT_GFP was a gift from Yong-Sun Bahn (Addgene plasmid # 92085 ; http://n2t.net/addgene:92085 ; RRID:Addgene_92085) -
For your References section:
Molecular Characterization of Adenylyl Cyclase Complex Proteins Using Versatile Protein-Tagging Plasmid Systems in Cryptococcus neoformans. So YS, Yang DH, Jung KW, Huh WK, Bahn YS. J Microbiol Biotechnol. 2017 Feb 28;27(2):357-364. doi: 10.4014/jmb.1609.09036. 10.4014/jmb.1609.09036 PubMed 27780958
