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StZapD(1-247)_pRSF_WT
(Plasmid #98986)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 98986 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pRSF
  • Total vector size (bp) 4500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Z-ring associated protein D
  • Species
    Salmonella typhimurium
  • Insert Size (bp)
    741
  • Mutation
    none
  • Promoter T7
  • Tag / Fusion Protein
    • 6X His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Transform into BL21(DE3) for protein expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    StZapD(1-247)_pRSF_WT was a gift from Hyung Ho Lee (Addgene plasmid # 98986)

  • For your References section:

    Structural and Biochemical Studies Reveal a Putative FtsZ Recognition Site on the Z-ring Stabilizer ZapD. Choi H, Min K, Mikami B, Yoon HJ, Lee HH. Mol Cells. 2016 Nov 30;39(11):814-820. Epub 2016 Nov 18. 10.14348/molcells.2016.0202 PubMed 27871169
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