pLV-shP2rx4-1026
(Plasmid
#99034)
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PurposeExpresses a shRNA targeting the rat P2rx4 gene
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99034 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLL3.7
- Backbone size w/o insert (bp) 7649
- Total vector size (bp) 7700
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Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameP2rx4 shRNA
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gRNA/shRNA sequenceGCACACTCACCAAGGCGTA
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SpeciesR. norvegicus (rat)
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GenBank IDNM_031594.1
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Entrez GeneP2rx4
- Promoter mouse U6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer U6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was generated as part of the NIAAA-funded INIA consortium.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-shP2rx4-1026 was a gift from Amy Lasek (Addgene plasmid # 99034 ; http://n2t.net/addgene:99034 ; RRID:Addgene_99034) -
For your References section:
Involvement of Purinergic P2X4 Receptors in Alcohol Intake of High-Alcohol-Drinking (HAD) Rats. Franklin KM, Hauser SR, Lasek AW, Bell RL, McBride WJ. Alcohol Clin Exp Res. 2015 Oct;39(10):2022-31. doi: 10.1111/acer.12836. Epub 2015 Sep 3. 10.1111/acer.12836 PubMed 26334550
