Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Analyze Sequence Guide

To analyze a sequence, paste your sequence into the box, then click Submit. You will have the following options:

  • Enter a new sequence link: Start over
  • Map and Features tab:
      This link will show a map of your sequence, with features and restriction sites listed below the map. Only commonly used enzymes are displayed. Use the links at the bottom to show or hide features.
  • Sequence tab:
    • FASTA: View your sequence in FASTA format (not numbered).
    • GenBank: View your sequence in GenBank format (features listed, numbered).
    • Reverse Complement: View the reverse complement of your sequence.
  • BLAST tab:
    • Determine what your sequence encodes by using BLASTN to search for a DNA match or BLASTX to search for a protein match.
  • Align tab:
    • Compare your sequence to another sequence.
  • Digest tab:
    • View restriction sites in your sequence. View unique cutters, non-cutters, and expected cut sites.
  • Translate tab:
    • View open reading frames in your sequence with common tags highlighted. Also see a translation of your sequence in all reading frames.