Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes.
Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild K, Bethune J
Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 90003 | pSF3-Flag-CBir-FRB_Myc-NBir-FKBP | Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP |
| 90004 | pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir | Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256] |
| 90008 | pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP | Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP |
| 90009 | pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir | Split-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBP |
| 90010 | pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e | Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e |
| 90011 | pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e | Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e |
| 90012 | pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP | Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP |
| 90013 | pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer | Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer |
| 90014 | pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C | Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C |
| 90015 | pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP | Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP |