A FRET biosensor reveals spatiotemporal activation and functions of aurora kinase A in living cells.
Bertolin G, Sizaire F, Herbomel G, Reboutier D, Prigent C, Tramier M
Nat Commun. 2016 Sep 14;7:12674. doi: 10.1038/ncomms12674.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 99875 | pET21-GFP-AURKA | Expression of GFP-AURKA for protein purification in bacteria |
| 99876 | pET21-GFP-AURKA-mCherry | Expression of GFP-AURKA-mCherry for protein purification in bacteria |
| 99877 | pAuroraA-GFP-AURKA | Expression of GFP-AURKA in mammalian cells |
| 99878 | pAuroraA-GFP-AURKA-mCherry | Expression of GFP-AURKA-mCherry in mammalian cells |
| 99879 | pCETN1-IRFP670 | Expression of CETN1-iRFP670 in mammalian cells |
| 99880 | pAuroraA-GFP-AURKA_K162M-mCherry | Expression of GFP-AURKA K162M-mCherry in cells |
| 99881 | pET21a-GFP-AURKA_K162M-mCherry | Expression of GFP-AURKA K162M-mCherry for protein purification in bacteria |