Ultrastructural localisation of protein interactions using conditionally stable nanobodies.
Ariotti N, Rae J, Giles N, Martel N, Sierecki E, Gambin Y, Hall TE, Parton RG
PLoS Biol. 2018 Apr 5;16(4):e2005473. doi: 10.1371/journal.pbio.2005473. eCollection 2018 Apr. PubMed Article
Ariotti N, Rae J, Giles N, Martel N, Sierecki E, Gambin Y, Hall TE, Parton RG
PLoS Biol. 2018 Apr 5;16(4):e2005473. doi: 10.1371/journal.pbio.2005473. eCollection 2018 Apr. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108874 | APEX2-csGBP | Expression of APEX2 fused to a conditionally stable nanobody against GFP |
| 108875 | mKate2-P2A-APEX2-csGBP | Expression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter) |
| 108876 | APEX2-csChBP | Expression of APEX2 fused to a conditionally stable nanobody against mCherry |
| 108877 | EGFP-P2A-APEX2-csChBP | Expression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter) |
| 108878 | APEX2-ChBP | Expression of APEX2 fused to a nanobody against mCherry |
| 108879 | EGFP-P2A-APEX2-ChBP | Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter) |
| 108880 | H2B-mCherry | Expression of human histone2B fused to mCherry (nuclear marker) |
| 108881 | nls-mCherry | Expression of nuclear localized mCherry |
| 108882 | pME-nls | Multisite gateway vector for 5' tagging with an N-terminal nuclear localisation sequence |
| 108883 | pME-H2B | Multisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation) |
| 108884 | p3E-mCherry | Multisite gateway vector for 3' tagging with mCherry |
| 108885 | pME-mCherry-NS | Multisite gateway vector for 5' tagging with mCherry |
| 108886 | mCherry-CaaX(Hras) | Expression of membrane localized mCherry |
| 108887 | mVenusN-Cavin1 | Expression of Cavin1 fused to the N-terminus of mVenus for bimolecular fluorescence complementation |
| 108888 | mVenusC-Cavin1 | Expression of Cavin1 fused to the C-terminus of mVenus for bimolecular fluorescence complementation |
| 108889 | mVenusN-Cavin3 | Expression of Cavin3 fused to the N-terminus of mVenus for bimolecular fluorescence complementation |
| 108890 | mVenusC-Cavin3 | Expression of Cavin3 fused to the C-terminus of mVenus for bimolecular fluorescence complementation |
| 108891 | p3E-csGBP | Multisite gateway vector for 3' tagging with a conditionally stable nanobody against GFP |
| 108892 | p3E-ChBP | Multisite gateway vector for 3' tagging with a nanobody against mCherry |
| 108893 | p3E-csChBP | Multisite gateway vector for 3' tagging with a conditionally stable nanobody against mCherry |
| 108894 | p3E-APEX2 | Multisite gateway vector for 3' tagging with APEX2 |
| 108895 | pME-EGFP-P2A-APEX2-NS | Multisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter) |
| 108896 | p3E-APEX2-P2A-EGFP | Multisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter) |