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Ultrastructural localisation of protein interactions using conditionally stable nanobodies.
Ariotti N, Rae J, Giles N, Martel N, Sierecki E, Gambin Y, Hall TE, Parton RG
PLoS Biol. 2018 Apr 5;16(4):e2005473. doi: 10.1371/journal.pbio.2005473. eCollection 2018 Apr.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
108874APEX2-csGBPExpression of APEX2 fused to a conditionally stable nanobody against GFP
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108875mKate2-P2A-APEX2-csGBPExpression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)
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108876APEX2-csChBPExpression of APEX2 fused to a conditionally stable nanobody against mCherry
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108877EGFP-P2A-APEX2-csChBPExpression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)
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108878APEX2-ChBPExpression of APEX2 fused to a nanobody against mCherry
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108879EGFP-P2A-APEX2-ChBPExpression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)
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108880H2B-mCherryExpression of human histone2B fused to mCherry (nuclear marker)
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108881nls-mCherryExpression of nuclear localized mCherry
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108882pME-nlsMultisite gateway vector for 5' tagging with an N-terminal nuclear localisation sequence
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108883pME-H2BMultisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation)
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108884p3E-mCherryMultisite gateway vector for 3' tagging with mCherry
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108885pME-mCherry-NSMultisite gateway vector for 5' tagging with mCherry
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108886mCherry-CaaX(Hras)Expression of membrane localized mCherry
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108887mVenusN-Cavin1Expression of Cavin1 fused to the N-terminus of mVenus for bimolecular fluorescence complementation
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108888mVenusC-Cavin1Expression of Cavin1 fused to the C-terminus of mVenus for bimolecular fluorescence complementation
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108889mVenusN-Cavin3Expression of Cavin3 fused to the N-terminus of mVenus for bimolecular fluorescence complementation
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108890mVenusC-Cavin3Expression of Cavin3 fused to the C-terminus of mVenus for bimolecular fluorescence complementation
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108891p3E-csGBPMultisite gateway vector for 3' tagging with a conditionally stable nanobody against GFP
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108892p3E-ChBPMultisite gateway vector for 3' tagging with a nanobody against mCherry
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108893p3E-csChBPMultisite gateway vector for 3' tagging with a conditionally stable nanobody against mCherry
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108894p3E-APEX2Multisite gateway vector for 3' tagging with APEX2
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108895pME-EGFP-P2A-APEX2-NSMultisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)
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108896p3E-APEX2-P2A-EGFPMultisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)
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Recombinant Antibodies from Article