Cryo-EM shows how dynactin recruits two dyneins for faster movement.

Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz A, Carter AP
Nature. 2018 Feb 7;554(7691):202-206. doi: 10.1038/nature25462. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
111657	His-ZZ-TEV-BICDR1-GFP	C-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.
111857	His-ZZ-TEV-SNAPf-BICD2N(1-400)	N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.
111858	His-ZZ-TEV-SNAPf-BICDR1	N-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.
111859	His-ZZ-TEV-BICDR1	Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.
111860	Hook3(1-522)-SNAPf-Psc-StrepII	C-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.
111861	Hook3(1-522)-GFP-Psc-StrepII	C-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.
111862	His-ZZ-TEV-BICDN(1-400)-GFP	C-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.