Insights into the allosteric regulation of Syk association with receptor ITAM, a multi-state equilibrium.
Feng C, Post CB
Phys Chem Chem Phys. 2016 Feb 17;18(8):5807-18. doi: 10.1039/c5cp05417f.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111269 | pET-30a(+)-Syk-tSH2 | express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3) |
| 111271 | pET-30a(+)-Syk-tSH2-PM | express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3) |
| 111272 | pET-30a(+)-Syk-tSH2-FX | express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3, in E.coli strain Rosetta 2 (DE3) |
| 111273 | pET-30a(+)-N-SH2+IA | express murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3) |
| 111274 | pET-30a(+)-N-SH2 | express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3) |