Enhancement of CRISPR-Cas9 induced precise gene editing by targeting histone H2A-K15 ubiquitination.

Bashir S, Dang T, Rossius J, Wolf J, Kuhn R
BMC Biotechnol. 2020 Oct 23;20(1):57. doi: 10.1186/s12896-020-00650-x. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
118997	pAAVS1-TLR6	Targeting vector for human AAVS1 with TLR6 reporter insert
118998	pU6Rosa-CAG-Cas9	Mammalian expression of Rosa26 sgRNA, Cas9 and BFP
118999	pTLR-donor	Repair template for the TLR reporter
119000	pTLR-donor-tetO	Repair template for the TLR reporter with 7 tet operator elements
119001	pTLR-donor-UAS	Repair template for the TLR reporter with 10 UAS binding sites for yeast Gal4
119002	pCAG-i53	Mammalian expression of i53 (53BP1) inhibitor and BFP
119003	pCAG-Rad18	Mammalian expression of human Rad18 and BFP
119004	pCAG-RNF169	Mammalian expression of human RNF169 and BFP
119005	pCAG-Rad18UBD	Mammalian expression of the human Rad18 Ubiquitin binding domain and BFP
119006	pCAG-RNF169UBD	Mammalian expression of the human RNF169 Ubiquitin binding domain and BFP
119007	pCAG-BRCA1	Mammalian expression of human BRCA1 and BFP
119008	pCAG-BRCA1-Rad18UBD	Mammalian expression of a fusion protein of human BRCA1 with human Rad18 Ubiquitin binding domain and BFP expression
119009	pCAG-BRCA1-RNF169UBD	Mammalian expression of a fusion protein of human BRCA1 with the human RNF169 Ubiquitin binding domain and BFP expression
119010	pCAG-TetR-Rad18UBD	Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression
119011	pCAG-TetR-RNF169UBD	Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression
119012	pU6Rosa-CAG-Gal4-Rad18UBD	Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression
119013	pCAG-Gal4-RNF169UBD	Mammalian expression of fusion protein of yeast Gal4 with human RNF169 Ubiquitin binding domain and BFP expression