Generating Single Cell-Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9.
Giuliano CJ, Lin A, Girish V, Sheltzer JM
Curr Protoc Mol Biol. 2019 Sep;128(1):e100. doi: 10.1002/cpmb.100.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120577 | LRG2.1-BFP | Lentiviral plasmid to co-express a guide RNA and EBFP. |
| 124770 | Lenti-Cas9-gRNA-GFP | 3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone |
| 124771 | LRG2.1-CyOFP1 | 3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1 |
| 124772 | LRG2.1-mOrange | 3rd generation lentiviral plasmid to co-express a guide RNA and mOrange |
| 124773 | LRG2.1-TagBFP2 | Lentiviral plasmid to co-express a guide RNA and TagBFP2 |
| 124774 | Lenti-Cas9-gRNA-TagBFP2 | 3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone |