Human ATG4 autophagy proteases counteract attachment of ubiquitin-like LC3/GABARAP proteins to other cellular proteins.
Agrotis A, von Chamier L, Oliver H, Kiso K, Singh T, Ketteler R
J Biol Chem. 2019 Jul 17. pii: AC119.009977. doi: 10.1074/jbc.AC119.009977.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129289 | pCMV 3xFLAG-LC3B Q116P G120 | Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells |
| 129290 | pDEST-CMV GFP-LC3B Q116P G120 | Expresses GFP-tagged deconjugation-resistant LC3B in mammalian cells |
| 129291 | pDONR223 LC3B Q116P G120 | Gateway entry clone encoding human deconjugation-resistant LC3B |
| 129292 | pDONR223 ATG3 WT | Gateway entry clone encoding wild-type human ATG3 with a stop codon |
| 129293 | pDONR223 ATG3 C264A | Gateway entry clone encoding human ATG3 C264A (catalytic inactive) |
| 129294 | pDONR223 ATG3 K243R | Gateway entry clone encoding human ATG3 K243R |
| 129295 | pDONR223 ATG3 allKR | Gateway entry clone encoding human ATG3 with all 22 lysine residues mutated to arginine |
| 129296 | pCMV 3xFLAG-ATG3 WT | Expresses 3xFLAG-ATG3 WT in mammalian cells |
| 129297 | pCMV 3xFLAG-ATG3 C264A | Expresses 3xFLAG-ATG3 C264A in mammalian cells |
| 129298 | pCMV 3xFLAG-ATG3 allKR | Expresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cells |
| 129299 | pCMV 3xFLAG-ATG3 1-13 KR | Expresses pCMV 3xFLAG-ATG3 1-13 KR (first thirteen lysines mutated to arginine) in mammalian cells |
| 129300 | pCMV 3xFLAG-ATG3 14-22 KR | Expresses pCMV 3xFLAG-ATG3 14-22 KR (last nine lysines mutated to arginine) in mammalian cells |
| 129301 | pCMV 3xFLAG-ATG3 K243R | Expresses pCMV 3xFLAG-ATG3 K243R in mammalian cells |
| 129302 | pCMV 3xFLAG-ATG3 K295R | Expresses pCMV 3xFLAG-ATG3 K295R in mammalian cells |