Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Functional Reconstitution of Intracellular Vesicle Fusion Using Purified SNAREs and Sec1/Munc18 (SM) Proteins.
Yu H, Crisman L, Stowell MHB, Shen J
Methods Mol Biol. 2019;1860:237-249. doi: 10.1007/978-1-4939-8760-3_15. PubMed Article

Plasmids from Article

ID Plasmid Purpose
135550pET-28a-His6-SUMO-Munc18-1wtExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin
135551pET-28a-His6-SUMO-VAMP2Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin
135552pET-28a-His6-SUMO-VAMP3Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin
135553pET-28a-His6-SUMO-VAMP8Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin
135554pFastBac HT JS-Munc18bExpress rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.
135555pFastBac HT JS-Munc18cExpress mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

Antibodies from Article