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High-Resolution Genome-Wide Occupancy in Candida spp. Using ChEC-seq.
Tebbji F, Khemiri I, Sellam A
mSphere. 2020 Oct 14;5(5). pii: 5/5/e00646-20. doi: 10.1128/mSphere.00646-20. PubMed Article

Plasmids from Article

ID Plasmid Purpose
162461pFA-MNase-CaURA3DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaURA3
162462pFA-MNase-CaHIS1DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-Ca HIS1
162463pFA-MNase-CaARG4DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaARG4
162465pFA-MNase-SAT1pFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNase
162556Cip-pAct1-MnaseNheI-MluI-digested 3xFLAG-MNase-SV40 fragment was cloned into the CIp-pACT1-CYC vector to ensure constitutive expression of MNase in C. albicans.
162557Cip-pAct1-Mnase-SAT1For the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1

Antibodies from Article