A Single Nucleotide Change in the polC DNA Polymerase III in Clostridium thermocellum Is Sufficient To Create a Hypermutator Phenotype.
Lanahan A, Zakowicz K, Tian L, Olson DG, Lynd LR
Appl Environ Microbiol. 2022 Jan 11;88(1):e0153121. doi: 10.1128/AEM.01531-21. Epub 2021 Oct 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174300 | pLT237 | Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance. |
| 174301 | pDGO186N-KS1 | Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene. |
| 174302 | pDGO186N-KS2 | Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene. |
| 174303 | pDGO186N-KS3 | Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene. |