Programmable eukaryotic protein synthesis with RNA sensors by harnessing ADAR.
Jiang K, Koob J, Chen XD, Krajeski RN, Zhang Y, Volf V, Zhou W, Sgrizzi SR, Villiger L, Gootenberg JS, Chen F, Abudayyeh OO
Nat Biotechnol. 2022 Oct 27. pii: 10.1038/s41587-022-01534-5. doi: 10.1038/s41587-022-01534-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182540 | pDY1243-Fluorescent RADARS | Express Fluorescent RADARS in bicistronic vector with mCherry as control gene |
| 193191 | pDY1314_RADARS_Luciferase | Express Gaussia Luciferase conditional upon RNA transcript |
| 193192 | pDY1173_ADAR1p150 | Express full length ADAR1p150 |
| 193193 | pDY1461_SERPINA1_CCA32_5avidity Cre | Express Cre conditional upon RNA transcript |
| 193194 | pDY1462_SERPINA1_CCA32_Caspase | Express Caspase conditional upon RNA transcript |
| 217490 | pKJ276 | RADARSv3 driving mNeonGreen |
| 217491 | pKJ275 | RADARSv3 driving luciferase |