Optimized fluorescent proteins for 4-color and photoconvertible live-cell imaging in Neurospora crassa.

Wang Z, Bartholomai BM, Loros JJ, Dunlap JC
Fungal Genet Biol. 2022 Dec 5:103763. doi: 10.1016/j.fgb.2022.103763. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
191561	csr-1_son-1_sGFP	Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.
191741	csr-1_son-1_mApple	Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.
191742	csr-1_son-1_mCherry	Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.
191743	csr-1_son-1_mNeonGreen	Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.
191744	csr-1_son-1_mRuby3	Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.
191745	csr-1_son-1_mScarlet	Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.
191746	csr-1_son-1_mTagBFP2	Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.
191747	csr-1_son-1_vsfGFP9	Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.
191748	csr-1_son-1_mNeptune2.5	Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.
191749	csr-1_son-1_iRFP670	Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.
191750	csr-1_hH1_mEos3.1_V5	Photoconvertible fluorescent nuclear reporter.
191751	csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2	Microtubule marker together with nuclear envelope marker driven by bidirectional promoter