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Addgene
Dorus Gadella Lab: Goedhart/Gadella plasmids 2022
Unpublished

Plasmids from Article

ID Plasmid Purpose
191453nanobody(AntiGFP)-HaloTag-iLIDLocalizing iLID with GFP-tagged proteins
191455pFPO-His-G-Ca-FLITSBacterial isolation of genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change
191456pFPO-His-mTurquoise2_T203YBacterial isolation of mTurquoise2 with a T203Y mutation
191457pFR-mScarletI-antiFRET-P2A-G-Ca-FLITSCompare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein
191458pFR-mScarletI-antiFRET-P2A-EGFPCompare the intensity of EGFP to mScarlet-I fluorescent protein
191459pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203YCompare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent protein
191460pFR-mScarletI-antiFRET-P2A-GCaMP3Compare the intensity of the genetically encoded calcium sensor (GECI) GCaMP3 to mScarlet-I fluorescent protein
191461pFR-mScarletI-antiFRET-P2A-jGCaMP7cCompare the intensity of the genetically encoded calcium sensor (GECI) jGCaMP7c to mScarlet-I fluorescent protein
1914624xmts-G-Ca-FLITSGenetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change
1914633xnls-G-Ca-FLITSGenetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change
191464ER-G-Ca-FLITS-KDELGenetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change
191465N1-G-Ca-FLITSGenetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change
191466pFR-mTq2-antiFRET-P2A-mCherryCompare the intensity of mCherry to mTurquoise2 fluorescent protein
191467pFR-mTq2-antiFRET-P2A-RCaMP1hCompare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent protein
191468pFR-mTq2-antiFRET-P2A-jRCaMP1bCompare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent protein
191469pFR-mTq2-antiFRET-P2A-K-GECO1Compare the intensity of the genetically encoded calcium sensor (GECI) K-GECO1 to mTurquoise2 fluorescent protein
191470pFR-mTq2-antiFRET-P2A-CH-GECO2.1Compare the intensity of the genetically encoded calcium sensor (GECI) CH-GECO2.1 to mTurquoise2 fluorescent protein
191471pFR-mTq2-antiFRET-P2A-R-GECO1Compare the intensity of the genetically encoded calcium sensor (GECI) R-GECO1 to mTurquoise2 fluorescent protein
191472pDRESS-mTq2-antiFRET-P2A2-linker_noSacINegative control for ratiometric comparison between red fluorescent proteins and sensors
191473pDRESS-mScarletI-antiFRET-P2A2-linker_noSacINegative control for ratiometric comparison between green fluorescent proteins and sensors

Antibodies from Article