RASopathy mutations provide functional insight into the BRAF cysteine-rich domain and reveal the importance of autoinhibition in BRAF regulation.
Spencer-Smith R, Terrell EM, Insinna C, Agamasu C, Wagner ME, Ritt DA, Stauffer J, Stephen AG, Morrison DK
Mol Cell. 2022 Nov 17;82(22):4262-4276.e5. doi: 10.1016/j.molcel.2022.10.016. Epub 2022 Nov 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194771 | CMV-BRAF-REG-Halo | Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag. |
| 194772 | CMV-NanoLuc-BRAF-CAT | Mammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag. |
| 202549 | pCMV-BRAF-REG-T241P-Halo | Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag. The REG domain contains the RASopathy mutation, T241P, within its cysteine-rich domain. |