Tuning Plant Promoters Using a Simple Split Luciferase Method to Assess Transcription Factor-DNA Interactions.
Cai YM, Witham S, Patron NJ
ACS Synth Biol. 2023 Nov 17;12(11):3482-3486. doi: 10.1021/acssynbio.3c00094. Epub 2023 Oct 19.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196141 | pEPYCeGM0001 | Gateway entry vector with Arabidopsis TGA1 CDS and c-terminal HiBiT tag |
| 196142 | pEPYCeGM0002 | Gateway entry vector with Arabidopsis TGA2 CDS and c-terminal HiBiT tag |
| 196143 | pEPYCeGM0009 | Gateway entry vector with Arabidopsis NLP6 CDS and c-terminal HiBiT tag |
| 196144 | pEPYCeGM0010 | Gateway entry vector with Arabidopsis NLP7 CDS and c-terminal HiBiT tag |
| 196145 | pEPYCeGM0011 | Gateway entry vector with Arabidopsis DREB26 CDS and c-terminal HiBiT tag |
| 196146 | pEPYCeGM0012 | Gateway entry vector with Arabidopsis ANAC032 CDS and c-terminal HiBiT tag |
| 196147 | pEPYCeGM0022 | Gateway entry vector with Arabidopsis ARF18 CDS and c-terminal HiBiT tag |
| 196148 | pEPYCdKN0001 | Gateway destination vector for protein expression of 9xHis_AtTGA1_HiBit |
| 196149 | pEPYCdKN0002 | Gateway destination vector for protein expression of 9xHis_AtTGA2_HiBit |
| 196150 | pEPYCdKN0009 | Gateway destination vector for protein expression of 9xHis_AtNLP6_HiBit |
| 196151 | pEPYCdKN0010 | Gateway destination vector for protein expression of 9xHis_AtNLP7_HiBit |
| 196152 | pEPYCdKN0011 | Gateway destination vector for protein expression of 9xHis_AtDREB26_HiBit |
| 196153 | pEPYCdKN0012 | Gateway destination vector for protein expression of 9xHis_AtNAC032_HiBit |
| 196154 | pEPYCdKN0022 | Gateway destination vector for protein expression of 9xHis_AtARF18_HiBit |
| 196155 | pEPYC0CM0515 | Phytobricks Level 0 part: MinSyn_301 |
| 196156 | pEPYC0CM0519 | Phytobricks Level 0 part: MinSyn_302 |
| 196157 | pEPYC0CM0520 | Phytobricks Level 0 part: MinSyn_303 |
| 196158 | pEPYC0CM0522 | Phytobricks Level 0 part: MinSyn_305 |
| 196159 | pEPYC0CM0513 | Phytobricks Level 0 part: MinSyn_308 |
| 196160 | pEPYC0CM0528 | Phytobricks Level 0 part: MinSyn_310 |
| 196161 | pEPYC0CM0529 | Phytobricks Level 0 part: MinSyn_311 |
| 196162 | pEPSW0CM0014 | Phytobricks Level 0 part: ANAC032 promoter 1kb upstream |
| 196163 | pEPSW0CM0020 | Phytobricks Level 0 part: AtNLP6 promoter 1kb upstream |
| 196164 | pEPSW0CM0018 | Phytobricks Level 0 part: AtARF18 promoter 1kb upstream |
| 196165 | pEPSW0CM0350 | Phytobricks Level 0 part: AtTGA1_CDS |
| 196166 | pEPYC1CB0597 | MoClo Level 1 luciferase reporter: MinSyn_301 reporter [MinSyn_301::TMV::LucF::flag::ocsT] |
| 196167 | pEPYC1CB0601 | MoClo Level 1 luciferase reporter: MinSyn_302 reporter [MinSyn_302::TMV::LucF::flag::ocsT] |
| 196168 | pEPYC1CB0602 | MoClo Level 1 luciferase reporter: MinSyn_303 reporter [MinSyn_303::TMV::LucF::flag::ocsT] |
| 196169 | pEPYC1CB0604 | MoClo Level 1 luciferase reporter: MinSyn_305 reporter [MinSyn_305::TMV::LucF::flag::ocsT] |
| 196170 | pEPYC1CB0595 | MoClo Level 1 luciferase reporter: MinSyn_308 reporter [MinSyn_308::TMV::LucF::flag::ocsT] |
| 196171 | pEPYC1CB0607 | MoClo Level 1 luciferase reporter: MinSyn_310 reporter [MinSyn_308::TMV::LucF::flag::ocsT] |
| 196172 | pEPYC1CB0608 | MoClo Level 1 luciferase reporter: MinSyn_311 reporter [MinSyn_308::TMV::LucF::flag::ocsT] |
| 196173 | pEPSW1KN0351 | AtTGA1 expression cassette: CaMV35S::TMV::AtTGA1::35sT |
| 196174 | pEPSW1KN0014 | MoClo Level 1 luciferase reporter: proANAC032::LucF::flag::notT |
| 196175 | pEPSW1KN0020 | MoClo Level 1 luciferase reporter: proNLP6::LucN::flag::notT |
| 196176 | pEPSW1KN0018 | MoClo Level 1 luciferase reporter: proARF18::LucN::flag::notT |
| 196177 | pEPSW1KN0035 | MoClo Level 1 luciferase reporter: NOS::TMV::LucN::flag::nosT |
| 196178 | pEPSW1KN0034 | MoClo Level 1 luciferase reporter: CaMV35S::TMV::LucF::flag::35sT |
| 196179 | pEPOR1CB0068 | YFP expression cassette: CaMV35S::TMV::YFP::35sT |
| 197517 | pEPSW0CM0022 | Phytobricks Level 0 part |
| 197518 | pEPSW0CM0024 | Phytobricks Level 0 part |
| 197519 | pEPSW0CM0025 | Phytobricks Level 0 part |
| 197520 | pEPSW0CM0027 | Phytobricks Level 0 part |
| 197521 | pEPSW0CM0029 | Phytobricks Level 0 part |
| 197522 | pEPSW0CM0030 | Phytobricks Level 0 part |
| 197523 | pEPSW0CM0031 | Phytobricks Level 0 part |
| 197524 | pEPSW0CM0032 | Phytobricks Level 0 part |
| 197526 | pEPSW0CM0112 | Phytobricks Level 0 part |
| 197528 | pEPSW0CM0073 | Phytobricks Level 0 part |
| 197529 | pEPSW0CM0074 | Phytobricks Level 0 part |
| 197530 | pEPSW0CM0075 | Phytobricks Level 0 part |
| 197531 | pEPSW0CM0076 | Phytobricks Level 0 part |
| 197533 | pEPOZ0CM0138 | Phytobricks Level 0 part |
| 197534 | pEPOZ0CM0139 | Phytobricks Level 0 part |
| 197535 | pEPOZ0CM0137 | Phytobricks Level 0 part |
| 197536 | pEPSW1KN0070 | MoClo Level 1 luciferase reporter |
| 197537 | pEPSW1KN0016 | MoClo Level 1 luciferase reporter |
| 197538 | pEPSW1KN0022 | MoClo Level 1 luciferase reporter |
| 197539 | pEPSW1KN0024 | MoClo Level 1 luciferase reporter |
| 197540 | pEPSW1KN0025 | MoClo Level 1 luciferase reporter |
| 197541 | pEPSW1KN0027 | Level 1 gene expression cassette |
| 197542 | pEPSW1KN0029 | Level 1 gene expression cassette |
| 197543 | pEPSW1KN0030 | Level 1 gene expression cassette |
| 197544 | pEPSW1KN0031 | Level 1 gene expression cassette |
| 197545 | pEPSW1KN0032 | Level 1 gene expression cassette |
| 197547 | pEPSW1KN0113 | Level 1 gene expression cassette |
| 197549 | pEPOZ1KN0142 | Level 1 gene expression cassette |
| 197550 | pEPOZ1KN0143 | Level 1 gene expression cassette |
| 197551 | pEPOZ1KN0144 | Level 1 gene expression cassette |
| 197552 | pEPOZ1KN0145 | Level 1 gene expression cassette |
| 197554 | pEPOZ1KN0147 | Level 1 gene expression cassette |
| 197555 | pEPOZ1KN0148 | Level 1 gene expression cassette |