Comprehensive mutagenesis maps the effect of all single-codon mutations in the AAV2 rep gene on AAV production.
Jain NK, Ogden PJ, Church GM
Elife. 2024 Mar 27;12:RP87730. doi: 10.7554/eLife.87730.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198014 | pCMV-AAV2cap | For high-level expression of the AAV2 cap gene, suitable for use with pCMV-Rep78/68 SSM library |
| 198015 | pCMV-AAV5cap | For high-level expression of the AAV5 cap gene, suitable for use with pCMV-Rep78/68 SSM library |
| 198016 | pCMV-AAV9cap | For high-level expression of the AAV9 cap gene, suitable for use with pCMV-Rep78/68 SSM library |
| 198050 | pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) | By supplying an AAV cap gene of interest in trans, researchers can use this library to investigate the effects of rep mutations on production of a wide range of AAV capsid serotypes |