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Local changes in microtubule network mobility instruct neuronal polarization and axon specification.

Burute M, Jansen KI, Mihajlovic M, Vermonden T, Kapitein LC
Sci Adv. 2022 Nov 4;8(44):eabo2343. doi: 10.1126/sciadv.abo2343. Epub 2022 Nov 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
193713pB80-2xFKBP-L-mCherry-CAAX Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP
193714pB80-hsKIF5B(1-353)GCN4-L-GFPExpression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353)
193715pB80-hsKIF5B(1-353)-GCN4-L-mCherryExpression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)
193716pB80-hsKIF5B(1-560)-L-GFPExpression of GFP tagged human kinesin-1 (KIF5B aa1-560)
193717pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRBRapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules
193718pB80-hsKIF5B(1-560)Rigor-L-2xHAExpression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules
193719pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRBRapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport
193720pB80-hsKIF5B(1-560)Rigor-L-mVenusExpression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules
193721pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport
193723LifeAct-mCherry Labeling microtubules in living mammalian cells

Antibodies from Article