Iain Cheeseman Lab: Cheeseman lab plasmids Nov 2023

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
211864	pNM220_AAVS1 integration helper	Cas9 cutting at AAVS1 safe harbor locus
211865	pGS96_EGFP(A206K)-I3-01 AAVS donor	I3-01 transgene integration at AAVS1 safe harbor locus
211866	pGS97_EGFP(A206K)-CENP-T(1-242)-I3-01 AAVS donor	CENP-T(1-242)-I3-01 transgene integration at AAVS1 safe harbor locus
211867	pGS101_EGFP(A206K)-CENP-T(1-242) AAVS donor	CENP-T(1-242) transgene integration at AAVS1 safe harbor locus
211869	pGS373_tdTomato-1xGCN4-T2A-BSD Lenti	1xGCN4pep transgene expression
211870	pGS374_tdTomato-2xGCN4-T2A-BSD Lenti	2xGCN4pep transgene expression
211871	pGS375_tdTomato-3xGCN4-T2A-BSD Lenti	3xGCN4pep transgene expression
211872	pGS376_tdTomato-4xGCN4-T2A-BSD Lenti	4xGCN4pep transgene expression
211873	pGS377_tdTomato-6xGCN4-T2A-BSD Lenti	6xGCN4pep transgene expression
211874	pGS378_tdTomato-8xGCN4-T2A-BSD Lenti	8xGCN4pep transgene expression
211875	pGS379_tdTomato-10xGCN4-T2A-BSD Lenti	10xGCN4pep transgene expression
211876	pGS380_tdTomato-12xGCN4-T2A-BSD Lenti	12xGCN4pep transgene expression
211877	pGS381_tdTomato-14xGCN4-T2A-BSD Lenti	14xGCN4pep transgene expression
211878	pGS382_tdTomato-16xGCN4-T2A-BSD Lenti	16xGCN4pep transgene expression
211879	pGS383_tdTomato-18xGCN4-T2A-BSD Lenti	18xGCN4pep transgene expression
211880	pGS395_myc-1xGCN4-T2A-BSD Lenti	1xGCN4pep transgene expression
211881	pGS396_myc-2xGCN4-T2A-BSD Lenti	2xGCN4pep transgene expression
211882	pGS397_myc-3xGCN4-T2A-BSD Lenti	3xGCN4pep transgene expression
211883	pGS398_myc-4xGCN4-T2A-BSD Lenti	4xGCN4pep transgene expression
211884	pGS399_myc-6xGCN4-T2A-BSD Lenti	6xGCN4pep transgene expression
211885	pGS400_myc-8xGCN4-T2A-BSD Lenti	8xGCN4pep transgene expression
211886	pGS401_myc-10xGCN4-T2A-BSD Lenti	10xGCN4pep transgene expression
211887	pGS402_myc-12xGCN4-T2A-BSD Lenti	12xGCN4pep transgene expression
211888	pGS403_scFv-GCN4-sfGFP-GB1-HRV3C-CENP-T[1-242 T11A, T85A] in AAVS donor	CENP-T[1-242] transgene integration at AAVS1 safe harbor locus
211889	pGS140_6xHis-EGFP-I3-01	Bacterial expression of I3-01
211890	pGS58_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)] - I3-01	Bacterial expression of CENP-T[1-242/3D]-I3-01
211891	pGS275_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)]	Bacterial expression of CENP-T[1-242/3D]