Structure and position-specific interactions of prion-like domains in transcription factor Efg1 phase separation.
Wang SH, Zheng T, Fawzi NL
bioRxiv [Preprint]. 2023 Nov 9:2023.11.09.566450. doi: 10.1101/2023.11.09.566450.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216395 | His-MBP-FL-Efg1 | Expresses MBP-tagged full length Efg1 |
| 216396 | Efg1-ΔDBD | Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 |
| 216397 | Efg1-N-PrLD | Expresses 6xHis-tagged N-terminal prion-like domains of Efg1 |
| 216398 | Efg1-C-PrLD | Expresses 6xHis-tagged C-terminal prion-like domains of Efg1 |
| 216399 | Efg1-DBD | Expresses 6xHis-tagged DBD of Efg1 |
| 216400 | Efg1-ΔDBD-A51P/A56P | Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56 to proline mutations |
| 216401 | Efg1-ΔDBD-Q443P | Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation |
| 216402 | Efg1-ΔDBD-A51P/A56P/Q443P | Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutations |
| 216403 | Efg1-ΔDBD-A51P/A56P/Q70P/Q443P | Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutations |
| 216404 | Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P | Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutations |
| 216405 | Efg1-ΔDBD-Y4S/Y8S/Y16S | Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutations |
| 216406 | Efg1-ΔDBD-Y64S/Y66S/Y69S | Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutations |
| 216407 | Efg1-ΔDBD-Y101S/Y104S/Y106S | Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutations |
| 216408 | Efg1-ΔDBD-Y115S/Y132S/Y139S | Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutations |
| 216409 | Efg1-ΔDBD-Y401S/Y404S/Y409S | Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutations |
| 216410 | Efg1-ΔDBD-Y450S/Y452S/Y456S | Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutations |
| 216411 | Efg1-ΔDBD-Y478S/Y482S/Y484S | Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutations |