Light-directed evolution of dynamic, multi-state, and computational protein functionalities.
Gligorovski V, Labagnara M, Scutteri L, Blackholm M, Moglich A, Mansouri N, Rahi SJ
Cell. 2026 Mar 6:S0092-8674(26)00167-4. doi: 10.1016/j.cell.2026.02.002.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227746 | pVG117 | Expresses a version of light-inducible transcription factor EL222 (Asp31Tyr, Val139Leu) under a strong constitutive PGK1pr |
| 227747 | pVG118 | Expresses a version of light-inducible transcription factor EL222 (Asp31Tyr) under a strong constitutive PGK1pr |
| 227748 | pVG119 | Expresses a version of light-inducible transcription factor EL222 (Val139Leu) under a strong constitutive PGK1pr |
| 227749 | pVG135 | Expresses a version of light-inducible transcription factor EL222 (Lys99Glu) under a strong constitutive PGK1pr |
| 227750 | pVG136 | Expresses a version of light-inducible transcription factor EL222 (Pro165Ser) under a strong constitutive PGK1pr |
| 227751 | pVG137 | Expresses a version of light-inducible transcription factor EL222 (Val201Leu) under a strong constitutive PGK1pr |
| 227752 | pVG120 | Expresses a version of light-inducible transcription factor EL222 (Asp89Tyr) under a strong constitutive PGK1pr |
| 227753 | pVG147 | Expresses a version of light-inducible transcription factor EL222 (Gly94Cys) under a strong constitutive PGK1pr |
| 227754 | pVG143 | Expresses a version of light-inducible transcription factor EL222 (Leu87Ile) under a strong constitutive PGK1pr |
| 227755 | pVG123 | Expresses a version of light-inducible transcription factor EL222 (Ala157Val) under a strong constitutive PGK1pr |
| 227756 | pVG286 | Expresses a version of light-inducible transcription factor EL222 (Gly80Glu) under a strong constitutive PGK1pr |
| 227757 | pVG121 | Expresses a version of light-inducible transcription factor EL222 (Gly82Val) under a strong constitutive PGK1pr |
| 227758 | pVG124 | Expresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1pr |
| 227759 | pVG150 | Expresses a version of light-inducible transcription factor EL222 (Ser137Asn) under a strong constitutive PGK1pr |
| 227760 | pVG144 | Expresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1pr |
| 227761 | pVG178 | Expresses a version of light-inducible transcription factor EL222 (Glu84Lys) under a strong constitutive PGK1pr |
| 227762 | pVG298 | Expresses a version of light-inducible transcription factor EL222 (Thr83Ala) under a strong constitutive PGK1pr |
| 227771 | pVG329 | For genomic replacement of YOR1 gene in budding yeast |
| 251208 | pVG173 | Expresses a version of light-inducible transcription factor EL222 (Gly80Arg) under a strong constitutive PGK1pr |