Kate Adamala Lab: CyanoConstruct: Simple platform for cyanobacterial expression construct assembly and translational tuning
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251363 | pDFC(P0) | Replicative expression plasmid (shuttle vector) backbone for CyanoConstruct system. |
| 251364 | pSIC(P0) | Backbone for construct integration in Synechocystis locus slr1311 for CyanoConstruct system. |
| 251365 | (P1)PA1lacO1 | IPTG-inducible Plac promoter variant with lacIq repressor and RiboJ |
| 251366 | (P2)RBSS1 | Synthetic RBS based on Synechocystis ribosome 30S anti-SD |
| 251367 | (P2)RBSS2 | RBS from C-phycocyanin beta gene in Synechococcus sp. PCC 7002 |
| 251368 | (P2)RBSS3 | RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis) |
| 251369 | (P2)RBSS4 | RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis) |
| 251370 | (P2)RBSS5 | RBS from rbcL gene; slr0090 (Synechocystis) |
| 251371 | (P2)RBSS6 | Synthetic RBS used in expression in Synechocystis |
| 251372 | (P2)RBSS7 | RBS originally used in pDF-lac [74] |
| 251373 | (P2)RBSB | RBS designed in silico [75] for high expression in E. coli |
| 251374 | (P3)syfp2 | Gene encoding yellow fluorescent protein variant |
| 251375 | (P3)Adh | Gene encoding aldehyde reductase slr1192 (Synechocystis) |
| 251376 | (P4)RBSS1 | Synthetic RBS based on Synechocystis ribosome 30S anti-SD |
| 251377 | (P4)RBSS2 | RBS from C-phycocyanin beta gene in Synechococcus sp. PCC 7002 |
| 251378 | (P4)RBSS3 | RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis) |
| 251379 | (P4)RBSS4 | RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis) |
| 251380 | (P4)RBSS5 | RBS from rbcL gene; slr0090 (Synechocystis) |
| 251381 | (P4)RBSS6 | Synthetic RBS used in expression in Synechocystis |
| 251382 | (P4)RBSS7 | RBS originally used in pDF-lac [74] |
| 251383 | (P4)RBSB | RBS designed in silico [75] for high expression in E. coli |
| 251384 | (P5)efe | Gene encoding Ethylene Forming Enzyme (Pseudomonas syringae) |
| 251385 | (P5)pdc | Gene encoding pyruvate decarboxylase (Zymomonas mobilis) |
| 251386 | (P6)RBSS1 | Synthetic RBS based on Synechocystis ribosome 30S anti-SD |
| 251387 | (P6)RBSS2 | RBS from C-phycocyanin beta gene in Synechococcus sp. PCC 7002 |
| 251388 | (P6)RBSS3 | RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis) |
| 251389 | (P6)RBSS4 | RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis) |
| 251390 | (P6)RBSS5 | RBS from rbcL gene; slr0090 (Synechocystis) |
| 251391 | (P6)RBSS6 | Synthetic RBS used in expression in Synechocystis |
| 251392 | (P6)RBSS7 | RBS originally used in pDF-lac [74] |
| 251393 | (P6)RBSB | RBS designed in silico [75] for high expression in E. coli |
| 251394 | (P6)TT | rrnB T1 terminator derived from pTrc99A |
| 251395 | (P7)Pk | Gene encoding pyruvate kinase (Enterococcus faecalis) |
| 251396 | (P8)TT | rrnB T1 terminator derived from pTrc99A |