mTORC1 senses lysosomal amino acids through an inside-out mechanism that requires the vacuolar H-ATPase.

Zoncu R, Bar-Peled L, Efeyan A, Wang S, Sancak Y, Sabatini DM
Science. 2011 Nov 4;334(6056):678-83. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
34611	LAMP1-mRFP-FLAG	3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag
87931	pRK5-FLAG-ATP6V0D1	FLAG-tagged overexpression
87932	pRK5-ATP6V1D-HAGST	C-term HA-GST tagged overexpression
88856	pRK5-myc-PAT1	myc-TAG overexpression