Versatile Recombinant SUMOylation System for the Production of SUMO-Modified Protein.
Weber AR, Schuermann D, Schar P
PLoS One. 2014 Jul 9;9(7):e102157. doi: 10.1371/journal.pone.0102157. eCollection 2014.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 52258 | pSUMO1 | Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2 |
| 52259 | pSUMO2 | Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2 |
| 52260 | pSUMO3 | Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2 |
| 52261 | pSA1 | Bacterial expression of human 6His-tagged SUMO1 and SUMO-E1 |
| 52262 | pSA2 | Bacterial expression of human 6His-tagged SUMO2 and SUMO-E1 |
| 52263 | pSA3 | Bacterial expression of human 6His-tagged SUMO3 and SUMO-E1 |
| 52264 | pSC-PreE2 | Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker |
| 52265 | pSC-IntE2 | Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linker |
| 52266 | pTG-mTDG | Bacterial expression of mouse TDG with C-terminal GST-tag |
| 52267 | pTG-mTDG-K341R | Bacterial expression of mouse TDG-K341R with C-terminal GST-tag |
| 52268 | pSC-mTDG-IntE2 | Bacterial expression of mouse TDG C-terminally fused to GyrA intein, SUMO-E2 and GST |
| 52269 | pSC-mTDG-K341R-IntE2 | Bacterial expression of mouse TDG-K341R C-terminally fused to GyrA intein, SUMO-E2 and GST |
| 52270 | pSC-hTDG-IntE2 | Bacterial expression of human TDG C-terminally fused to GyrA intein, SUMO-E2 and GST |
| 52271 | pSC-hTDG-K330A-IntE2 | Bacterial expression of human TDG-K330A C-terminally fused to GyrA intein, SUMO-E2 and GST |
| 52272 | pSC-hTDG-PreE2 | Bacterial expression of human TDG C-terminally fused to SUMO-E2 and GST |
| 52273 | pSC-hTDG-K330A-PreE2 | Bacterial expression of human TDG-K330A C-terminally fused to SUMO-E2 and GST |
| 52274 | pGEX-hXRCC1 | Bacterial expression of human XRCC1 with N-terminal GST-tag |
| 52275 | pGEX-hXRCC1-K176R | Bacterial expression of human XRCC1-K176R with N-terminal GST-tag |
| 52276 | pSC-hXRCC1-IntE2 | Bacterial expression of human XRCC1 C-terminally fused to GyrA intein, SUMO-E2 and GST |
| 52277 | pSC-hXRCC1-K176R-IntE2 | Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein, SUMO-E2 and GST |
| 52278 | pSC-hXRCC1-PreE2 | Bacterial expression of human XRCC1 C-terminally fused to SUMO-E2 and GST |
| 52279 | pSC-hXRCC1-K176R-PreE2 | Bacterial expression of human XRCC1-K176R C-terminally fused to SUMO-E2 and GST |
| 52280 | pTXG | Bacterial expression of TARGET proteins C-terminally fused to GyrA intein and GST |
| 52281 | pTXG-hTDG | Bacterial expression of human TDG C-terminally fused to GyrA intein and GST |
| 52282 | pTXG-hTDG-K330A | Bacterial expression of human TDG-K330A C-terminally fused to GyrA intein and GST |
| 52283 | pTXG-hXRCC1 | Bacterial expression of human XRCC1 C-terminally fused to GyrA intein and GST |
| 52284 | pTXG-hXRCC1-K176R | Bacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein and GST |