GoldenBraid 2.0 E. coli Kit
(Kit #
1000000284
)
Depositing Lab: Christophe Herman
A modular cloning toolkit adhering to the GoldenBraid 2.0 standard. This toolkit is designed for use in E. coli and related enteric bacteria.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
GoldenBraid2.0 E. coli: a comprehensive and characterized toolkit for enterics. Cooke MB, Welch KT, Ramirez LD, Wen AX, Marciano DC, Herman C. Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf015. doi: 10.1093/synbio/ysaf015. eCollection 2025. PubMed (Link opens in a new window) Article (Link opens in a new window)
Description
The GoldenBraid 2.0 E. coli Toolkit is a comprehensive modular cloning resource designed to facilitate genetic engineering in Escherichia coli. Built on the GoldenBraid 2.0 Type IIS assembly platform, the toolkit enables rapid and standardized construction of plasmid-borne and chromosomally integrated genetic circuits through the reuse of interchangeable DNA parts. The collection includes 192 pre-assembled genetic parts for gene expression, protein tagging, and strain engineering, along with specialized cloning strains that streamline construct assembly and genome modification.
Figure 1: The GoldenBraid 2.0 E. coli toolkit is a modular cloning toolkit designed for use in E. coli and related enteric bacteria. The toolkit is designed to enable the “domestication” of genetic parts into the GoldenBraid 2.0 assembly standard, the assembly of these parts into transcription units which are functional in these species, and the combination of these units together into genetic constructs of arbitrary size. This toolkit is composed of a set of standardized E. coli vectors and genetic parts arranged in a “grammatical” standard optimized for the generation of E. coli genetic constructs.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
"The GoldenBraid 2.0 E. coli Kit was a gift from Christophe Herman (Addgene kit #1000000284)."
For your Reference section:
GoldenBraid2.0 E. coli: a comprehensive and characterized toolkit for enterics. Cooke MB, Welch KT, Ramirez LD, Wen AX, Marciano DC, Herman C. Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf015. doi: 10.1093/synbio/ysaf015. eCollection 2025. PubMed (Link opens in a new window) Article (Link opens in a new window)
GoldenBraid 2.0 E. coli Kit - #1000000284
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right, as well as lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Kanamycin | |
| Ampicillin | |
| Chloramphenicol |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| Plate 1 / A / 1 | pBSKa1_Red |
|
| Plate 1 / A / 2 | pBSKa2_Red |
|
| Plate 1 / A / 3 | pR6Ka1_Red |
|
| Plate 1 / A / 4 | pR6Ka2_Red |
|
| Plate 1 / A / 5 | pBSKa1_FRTampFRT |
|
| Plate 1 / A / 6 | pBSKa1_FRTkanFRT |
|
| Plate 1 / A / 7 | pBSKa1_FRTcamFRT |
|
| Plate 1 / A / 8 | pBSKa1_FRTtetFRT |
|
| Plate 1 / A / 9 | pBSKa1_FRTspcFRT |
|
| Plate 1 / A / 10 | pBSKa2_FRTampFRT |
|
| Plate 1 / A / 11 | pBSKa2_FRTkanFRT |
|
| Plate 1 / A / 12 | pBSKa2_FRTcamFRT |
|
| Plate 1 / B / 1 | pBSKa2_FRTtetFRT |
|
| Plate 1 / B / 2 | pBSKa2_FRTspcFRT |
|
| Plate 1 / B / 3 | pBSKa1_Stuffer1 |
|
| Plate 1 / B / 4 | pBSKa2_Stuffer1 |
|
| Plate 1 / B / 5 | pBSKa1_Stuffer2 |
|
| Plate 1 / B / 6 | pBSKa2_Stuffer2 |
|
| Plate 1 / B / 7 | p15Aa1_Blue |
|
| Plate 1 / B / 8 | p15Aa2_Blue |
|
| Plate 1 / B / 9 | p15Ao1_Blue |
|
| Plate 1 / B / 10 | p15Ao2_Blue |
|
| Plate 1 / B / 11 | p15Aa1_Red |
|
| Plate 1 / B / 12 | p15Aa2_Red |
|
| Plate 1 / C / 1 | p15Ao1_Red |
|
| Plate 1 / C / 2 | p15Ao2_Red |
|
| Plate 1 / C / 3 | pMB1a1_Blue |
|
| Plate 1 / C / 4 | pMB1a2_Blue |
|
| Plate 1 / C / 5 | pMB1o1_Blue |
|
| Plate 1 / C / 6 | pMB1o2_Blue |
|
| Plate 1 / C / 7 | pMB1a1_Red |
|
| Plate 1 / C / 8 | pMB1a2_Red |
|
| Plate 1 / C / 9 | pMB1o1_Red |
|
| Plate 1 / C / 10 | pMB1o2_Red |
|
| Plate 1 / C / 11 | pSC101TSa1_Blue |
|
| Plate 1 / C / 12 | pSC101TSa2_Blue |
|
| Plate 1 / D / 1 | pSC101TSo1_Blue |
|
| Plate 1 / D / 2 | pSC101TSo2_Blue |
|
| Plate 1 / D / 3 | pSC101TSa1_Red |
|
| Plate 1 / D / 4 | pSC101TSa2_Red |
|
| Plate 1 / D / 5 | pSC101TSo1_Red |
|
| Plate 1 / D / 6 | pSC101TSo2_Red |
|
| Plate 1 / D / 7 | ptetgRNA-FRT |
|
| Plate 1 / D / 8 | pUPD3_Red |
|
| Plate 1 / D / 9 | pBSKo1_Red |
|
| Plate 1 / D / 10 | pBSKo2_Red |
|
| Plate 1 / D / 11 | pR6Ko1_Red |
|
| Plate 1 / D / 12 | pR6Ko2_Red |
|
| Plate 1 / E / 1 | pUPD3_FULLUNIT_Stuffer1 |
|
| Plate 1 / E / 2 | pUPD3_FULLUNIT_Stuffer2 |
|
| Plate 1 / E / 3 | pUPD3_FULLPRM_Stuffer3 |
|
| Plate 1 / E / 4 | pUPD3_FULLPRM_Stuffer4 |
|
| Plate 1 / E / 5 | pUPD3_PARTPRM_Stuffer5 |
|
| Plate 1 / E / 6 | pUPD3_PARTPRM_Stuffer6 |
|
| Plate 1 / E / 7 | pUPD3_RBS_Stuffer7 |
|
| Plate 1 / E / 8 | pUPD3_RBS_Stuffer8 |
|
| Plate 1 / E / 9 | pUPD3_CDS_Stuffer9 |
|
| Plate 1 / E / 10 | pUPD3_CDS_Stuffer10 |
|
| Plate 1 / E / 11 | pUPD3_FULLTRM_Stuffer11 |
|
| Plate 1 / E / 12 | pUPD3_FULLTRM_Stuffer12 |
|
| Plate 1 / F / 1 | pUPD3_RTAGTRM_Stuffer13 |
|
| Plate 1 / F / 2 | pUPD3_RTAGTRM_Stuffer14 |
|
| Plate 1 / F / 3 | pUPD3_DTAGTRM_Stuffer15 |
|
| Plate 1 / F / 4 | pUPD3_DTAGTRM_Stuffer16 |
|
| Plate 1 / F / 5 | pUPD3_RDTAGTRM_Stuffer17 |
|
| Plate 1 / F / 6 | pUPD3_RDTAGTRM_Stuffer18 |
|
| Plate 1 / F / 7 | pBSKo1_Stuffer1_Stuffer2 |
|
| Plate 1 / F / 8 | pBSKo2_Stuffer2_Stuffer1 |
|
| Plate 1 / F / 9 | pR6Ko1_FRTampFRT |
|
| Plate 1 / F / 10 | pR6Ko1_FRTkanFRT |
|
| Plate 1 / F / 11 | pR6Ko1_FRTcamFRT |
|
| Plate 1 / F / 12 | pR6Ko1_FRTtetFRT |
|
| Plate 1 / G / 1 | pR6Ko1_FRTspcFRT |
|
| Plate 1 / G / 2 | pR6Ko2_FRTtetFRT |
|
| Plate 1 / G / 3 | pR6Ko2_FRTcamFRT |
|
| Plate 1 / G / 4 | pR6Ko2_FRTkanFRT |
|
| Plate 1 / G / 5 | pR6Ko2_FRTampFRT |
|
| Plate 1 / G / 6 | pR6Ko2_FRTspcFRT |
|
| Plate 1 / G / 7 | pUPD3_FULLPRM_Pem7 |
|
| Plate 1 / G / 8 | pUPD3_FULLPRM_lacI_Plac |
|
| Plate 1 / G / 9 | pUPD3_FULLPRM_Plac |
|
| Plate 1 / G / 10 | pUPD3_FULLPRM_araC_Para |
|
| Plate 1 / G / 11 | pUPD3_FULLPRM_Para |
|
| Plate 1 / G / 12 | pUPD3_FULLPRM_tetR_Ptet |
|
| Plate 1 / H / 1 | pUPD3_FULLPRM_Ptet |
|
| Plate 1 / H / 2 | pUPD3_FULLPRM_cI857_PR |
|
| Plate 1 / H / 3 | pUPD3_FULLPRM_PR |
|
| Plate 1 / H / 4 | pUPD3_FULLPRM_PT7 |
|
| Plate 1 / H / 5 | pUPD3_FULLPRM_PT7lac |
|
| Plate 1 / H / 6 | pUPD3_PARTPRM_Pem7 |
|
| Plate 1 / H / 7 | pUPD3_PARTPRM_lacI_Plac |
|
| Plate 1 / H / 8 | pUPD3_PARTPRM_Plac |
|
| Plate 1 / H / 9 | pUPD3_PARTPRM_araC_Para |
|
| Plate 1 / H / 10 | pUPD3_PARTPRM_Para |
|
| Plate 1 / H / 11 | pUPD3_PARTPRM_tetR_Ptet |
|
| Plate 1 / H / 12 | pUPD3_PARTPRM_Ptet |
|
| Plate 2 / A / 1 | pUPD3_PARTPRM_cI857_PR |
|
| Plate 2 / A / 2 | pUPD3_PARTPRM_PR |
|
| Plate 2 / A / 3 | pUPD3_PARTPRM_PT7 |
|
| Plate 2 / A / 4 | pUPD3_PARTPRM_PT7lac |
|
| Plate 2 / A / 5 | pUPD3_PARTPRM_J23100 |
|
| Plate 2 / A / 6 | pUPD3_PARTPRM_J23101 |
|
| Plate 2 / A / 7 | pUPD3_PARTPRM_J23102 |
|
| Plate 2 / A / 8 | pUPD3_PARTPRM_J23103 |
|
| Plate 2 / A / 9 | pUPD3_PARTPRM_J23104 |
|
| Plate 2 / A / 10 | pUPD3_PARTPRM_J23105 |
|
| Plate 2 / A / 11 | pUPD3_PARTPRM_J23106 |
|
| Plate 2 / A / 12 | pUPD3_PARTPRM_J23107 |
|
| Plate 2 / B / 1 | pUPD3_PARTPRM_J23108 |
|
| Plate 2 / B / 2 | pUPD3_PARTPRM_J23109 |
|
| Plate 2 / B / 3 | pUPD3_PARTPRM_J23110 |
|
| Plate 2 / B / 4 | pUPD3_PARTPRM_J23111 |
|
| Plate 2 / B / 5 | pUPD3_PARTPRM_J23113 |
|
| Plate 2 / B / 6 | pUPD3_PARTPRM_J23114 |
|
| Plate 2 / B / 7 | pUPD3_PARTPRM_J23115 |
|
| Plate 2 / B / 8 | pUPD3_PARTPRM_J23116 |
|
| Plate 2 / B / 9 | pUPD3_PARTPRM_J23117 |
|
| Plate 2 / B / 10 | pUPD3_PARTPRM_J23118 |
|
| Plate 2 / B / 11 | pUPD3_PARTPRM_J23119 |
|
| Plate 2 / B / 12 | pUPD3_RBS_J61100 |
|
| Plate 2 / C / 1 | pUPD3_RBS_J61101 |
|
| Plate 2 / C / 2 | pUPD3_RBS_J61102 |
|
| Plate 2 / C / 3 | pUPD3_RBS_J61103 |
|
| Plate 2 / C / 4 | pUPD3_RBS_J61104 |
|
| Plate 2 / C / 5 | pUPD3_RBS_J61105 |
|
| Plate 2 / C / 6 | pUPD3_RBS_J61106 |
|
| Plate 2 / C / 7 | pUPD3_RBS_J61107 |
|
| Plate 2 / C / 8 | pUPD3_RBS_J61108 |
|
| Plate 2 / C / 9 | pUPD3_RBS_J61109 |
|
| Plate 2 / C / 10 | pUPD3_RBS_J61110 |
|
| Plate 2 / C / 11 | pUPD3_RBS_J61111 |
|
| Plate 2 / C / 12 | pUPD3_RBS_J61112 |
|
| Plate 2 / D / 1 | pUPD3_RBS_J61113 |
|
| Plate 2 / D / 2 | pUPD3_RBS_J61114 |
|
| Plate 2 / D / 3 | pUPD3_RBS_J61115 |
|
| Plate 2 / D / 4 | pUPD3_RBS_J61116 |
|
| Plate 2 / D / 5 | pUPD3_RBS_J61117 |
|
| Plate 2 / D / 6 | pUPD3_RBS_J61118 |
|
| Plate 2 / D / 7 | pUPD3_RBS_J61119 |
|
| Plate 2 / D / 8 | pUPD3_RBS_J61120 |
|
| Plate 2 / D / 9 | pUPD3_RBS_J61121 |
|
| Plate 2 / D / 10 | pUPD3_RBS_J61122 |
|
| Plate 2 / D / 11 | pUPD3_RBS_J61123 |
|
| Plate 2 / D / 12 | pUPD3_RBS_J61124 |
|
| Plate 2 / E / 1 | pUPD3_RBS_J61125 |
|
| Plate 2 / E / 2 | pUPD3_RBS_J61126 |
|
| Plate 2 / E / 3 | pUPD3_RBS_J61127 |
|
| Plate 2 / E / 4 | pUPD3_RBS_J61128 |
|
| Plate 2 / E / 5 | pUPD3_RBS_J61129 |
|
| Plate 2 / E / 6 | pUPD3_RBS_J61130 |
|
| Plate 2 / E / 7 | pUPD3_RBS_J61131 |
|
| Plate 2 / E / 8 | pUPD3_RBS_J61132 |
|
| Plate 2 / E / 9 | pUPD3_RBS_J61133 |
|
| Plate 2 / E / 10 | pUPD3_RBS_J61134 |
|
| Plate 2 / E / 11 | pUPD3_RBS_J61135 |
|
| Plate 2 / E / 12 | pUPD3_RBS_J61136 |
|
| Plate 2 / F / 1 | pUPD3_RBS_J61137 |
|
| Plate 2 / F / 2 | pUPD3_RBS_J61138 |
|
| Plate 2 / F / 3 | pUPD3_RBS_J61139 |
|
| Plate 2 / F / 4 | pUPD3_RBS_RBS1 |
|
| Plate 2 / F / 5 | pUPD3_RBS_RBS2 |
|
| Plate 2 / F / 6 | pUPD3_RBS_RBS3 |
|
| Plate 2 / F / 7 | pUPD3_RBS_RBS4 |
|
| Plate 2 / F / 8 | pUPD3_RBS_RBS5 |
|
| Plate 2 / F / 9 | pUPD3_RBS_RBS6 |
|
| Plate 2 / F / 10 | pUPD3_RBS_RBS7 |
|
| Plate 2 / F / 11 | pUPD3_RBS_RBS8 |
|
| Plate 2 / F / 12 | pUPD3_RBS_RBS9 |
|
| Plate 2 / G / 1 | pUPD3_RBS_RBS10 |
|
| Plate 2 / G / 2 | pUPD3_RBS_RBS11 |
|
| Plate 2 / G / 3 | pUPD3_RBS_RBS12 |
|
| Plate 2 / G / 4 | pUPD3_RBS_RBS13 |
|
| Plate 2 / G / 5 | pUPD3_RBS_RBS14 |
|
| Plate 2 / G / 6 | pUPD3_RBS_RBS15 |
|
| Plate 2 / G / 7 | pUPD3_RBS_RBS16 |
|
| Plate 2 / G / 8 | pUPD3_RBS_RBS17 |
|
| Plate 2 / G / 9 | pUPD3_RBS_RBS18 |
|
| Plate 2 / G / 10 | pUPD3_RBS_RBS19 |
|
| Plate 2 / G / 11 | pUPD3_RBS_RBS20 |
|
| Plate 2 / G / 12 | pUPD3_RBS_RBS21 |
|
| Plate 2 / H / 1 | pUPD3_RBS_RBS22 |
|
| Plate 2 / H / 2 | pUPD3_RBS_RBS23 |
|
| Plate 2 / H / 3 | pUPD3_RBS_RBS24 |
|
| Plate 2 / H / 4 | pUPD3_RBS_RBS25 |
|
| Plate 2 / H / 5 | pUPD3_RBS_RBS26 |
|
| Plate 2 / H / 6 | pUPD3_RBS_RBS27 |
|
| Plate 2 / H / 7 | pUPD3_RBS_RBS28 |
|
| Plate 2 / H / 8 | pUPD3_CDS_lacI |
|
| Plate 2 / H / 9 | pUPD3_CDS_araC |
|
| Plate 2 / H / 10 | pUPD3_CDS_tetR |
|
| Plate 2 / H / 11 | pUPD3_CDS_cI857 |
|
| Plate 2 / H / 12 | pUPD3_CDS_T7pol |
|