We narrowed to 3,233 results for: multiplex

1. pYPQ-dAaCas12b-Act3.0

   Plasmid

   #158413

   Purpose

   CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-RTP1S-Integration

   Plasmid

   #129458

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses RTP1S

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   RTP1 (Rtp1 Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Removal of the first 36 amino acids of the protein

   Promoter

   TRE (Tet-On)

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Anti-Pan-Shank [N23B/49R]

   Plasmid

   #128637

   Purpose

   Mammalian Expression Plasmid of anti-Pan-Shank. Derived from hybridoma N23B/49.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-Pan-Shank Pan recombinant mouse monoclonal antibody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLQ10714

   Plasmid

   #183959

   Purpose

   U6-crKlf4-CAG-hyperdCas12a-miniVPR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Inserts

   HyperdCas12a
   crRNA targeting Klf4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D832A, D156R, E292R, D235R, D350R

   Promoter

   CAG and U6

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65

   Plasmid

   #104566

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1(D832A)-p65
   

   Use

   Tags

   3x HA and NLS

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Anti-S100A5 [N176A/35.1R]

   Plasmid

   #114560

   Purpose

   Mammalian Expression Plasmid of anti-S100A5 (Rat). Derived from hybridoma N176A/35.1.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-S100A5 (Rattus norvegicus) recombinant mouse monoclonal antibody (S100a5 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   dual CMV

   Available since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode

   Plasmid

   #170254

   Purpose

   Barcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   A662C, N664C, V667L

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGW045 AAV-CRISPRa base vector pAAV_OE-U6sgbbSapI(MS2)-EF1a-MPH-WPRE

   Plasmid

   #192161

   Purpose

   AAV-CRISPRa base vector

   Depositor

   Sidi Chen

   Article

   Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode

   Plasmid

   #170222

   Purpose

   Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   R24C

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)

    Plasmid

    #104568

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1(D832A)-DmrA(x1)
    

    Use

    Tags

    3x FLAG, 3x HA, and NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Anti-TrpV3 [N15/4R]

    Plasmid

    #177501

    Purpose

    Mammalian Expression Plasmid of anti-TrpV3 (Rat). Derived from hybridoma N15/4.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-TrpV3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv3 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Anti-Pan-Ankyrin [N388A/60R]

    Plasmid

    #128640

    Purpose

    Mammalian Expression Plasmid of anti-Pan-Ankyrin. Derived from hybridoma N388A/60.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Pan-Ankyrin Pan recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Anti-VGlut3 [N34/34.2R]

    Plasmid

    #114494

    Purpose

    Mammalian Expression Plasmid of anti-VGlut3 (Rat). Derived from hybridoma N34/34.2.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-VGlut3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc17a8 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. sgCRY2

    Plasmid

    #197457

    Purpose

    Expresses sgRNA(CRY2 gene) in mammalian cells

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgCRY2-1-hU6-sgCRY2-2 (Cry2 Mouse)
    

    Use

    AAV

    Tags

    mCherry (K14E, N210D, E249G)

    Expression

    Mutation

    Promoter

    U6, hSyn

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode

    Plasmid

    #170217

    Purpose

    Barcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CASP8 (CASP8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    C360A

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)

    Plasmid

    #104569

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1(D832A)-DmrA(x2)
    

    Use

    Tags

    3x FLAG, 3x HA, and NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGW05 AAV-CRISPRa base vector pAAV-OE_U6-Lib(MS2)-EF1a-MPH-sPA

    Plasmid

    #192159

    Purpose

    AAV-CRISPRa base vector

    Depositor

    Sidi Chen

    Article

    Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Anti-Pan-SAPAP [N127/31.1R]

    Plasmid

    #114529

    Purpose

    Mammalian Expression Plasmid of anti-Pan-SAPAP (Rat). Derived from hybridoma N127/31.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Pan-SAPAP (Rattus norvegicus) recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Anti-SVOP [N356/23R]

    Plasmid

    #114499

    Purpose

    Mammalian Expression Plasmid of anti-SVOP (Rat). Derived from hybridoma N356/23.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-SVOP (Rattus norvegicus) recombinant mouse monoclonal antibody (Svop Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode

    Plasmid

    #170225

    Purpose

    Barcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    ER (ESR1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Y537S

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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