Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 4,470 results for: try

Clear keywords
Showing: 2621 - 2640 of 4470 results

  1. pYPQ289-RVR

    Plasmid
    #138127
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoter
    Depositor
    Yiping Qi
    Publication
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    ErCas12a RVR variant
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    D513R, K519V and N523R
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138127-plasmid-map-sequence-id-272088

    Enlarge

  3. KG#797

    Plasmid
    #110916
    Purpose
    Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons
    Depositor
    Kenneth Miller
    Publication
    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:
    Inserts
    unc-129 promoter
    STRN-1 (Sentryn)-GFP
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    Tags
    GFP (contains 3 artificial introns)
    Expression
    Bacterial
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110916-plasmid-map-sequence-id-212580

    Enlarge

  5. RON2-COMP-blac-flag-his

    Plasmid
    #110962
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Publication
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    rhoptry neck protein 2 (RON2) (PF3D7_1452000 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    110962-plasmid-map-sequence-id-223581

    Enlarge

  6. KG#901

    Plasmid
    #110936
    Purpose
    Expresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animal
    Depositor
    Kenneth Miller
    Publication
    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:
    Inserts
    mig-13 promoter
    Emerald-RAB-3
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110936-plasmid-map-sequence-id-216868

    Enlarge

  10. pFP.R400

    Plasmid
    #138175
    Purpose
    Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)
    Depositor
    Baojun Wang
    Publication
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138175-plasmid-map-sequence-id-296599

    Enlarge

  11. pFP.R271

    Plasmid
    #138220
    Purpose
    Expresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Publication
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(IDE).SspDnaX.(CHT)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138220-plasmid-map-sequence-id-296583

    Enlarge

  12. pFP.R365

    Plasmid
    #138235
    Purpose
    Expresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Publication
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(HGK).EP-Pri*.(TEV)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138235-plasmid-map-sequence-id-273528

    Enlarge

  13. pFP.R375

    Plasmid
    #138245
    Purpose
    Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)
    Depositor
    Baojun Wang
    Publication
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138245-plasmid-map-sequence-id-273498

    Enlarge

  14. pFP.R401

    Plasmid
    #138222
    Purpose
    Expresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Publication
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(IGC).TerThyX*.(SFD)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138222-plasmid-map-sequence-id-273515

    Enlarge

  15. pBad-HPII TAG415

    Plasmid
    #105847
    Purpose
    expresses E. coli HPII catalase with TAG stop codon at site 415 and N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Publication
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 415
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105847-plasmid-map-sequence-id-205555

    Enlarge

  16. pBad-HPII TAG348

    Plasmid
    #105844
    Purpose
    expresses E. coli HPII catalase with TAG stop codon at site 348 and N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Publication
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 348
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105844-plasmid-map-sequence-id-205537

    Enlarge

  17. pBad-HPII TAG283

    Plasmid
    #105843
    Purpose
    expresses E. coli HPII catalase with TAG stop codon at site 283 and N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Publication
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 283
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105843-plasmid-map-sequence-id-205536

    Enlarge

  19. pAAV-Ef1a-FAS-GCaMP6f-WPRE

    Plasmid
    #141237
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Publication
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    GCaMP6f
    Use
    AAV and Cre/Lox
    Tags
    6XHIS and Xpress
    Promoter
    Human elongation factor-1 alpha (EF-1 alpha)
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    141237-plasmid-map-sequence-id-431695

    Enlarge

Showing: 2621 - 2640 of 4470 results