We narrowed to 9,753 results for: try

1. pENTR223-EBNA3A

   Plasmid

   #198575

   Purpose

   Gateway Entry Clone

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   EBNA3A (EBNA-3A Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Mutation

   None (wildtype)

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20944

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   H134R

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pEGFP-C1-Nup35-mut

   Plasmid

   #87341

   Purpose

   To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP35 (NUP35 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   shRNA resistant changes

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-SARS-CoV-2-E

   Plasmid

   #215814

   Purpose

   Expresses SARS-CoV-2 Envelope protein in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 Envelope (E )
   

   Expression

   Mammalian

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-SARS-CoV-2-M

   Plasmid

   #215813

   Purpose

   Expresses SARS-CoV-2 Membrane protein in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 Membrane (M )
   

   Expression

   Mammalian

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-SARS-CoV-2-S (D614G)

   Plasmid

   #215838

   Purpose

   Expresses SARS-CoV-2 Spike protein in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 Spike (S )
   

   Expression

   Mammalian

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMX-mTAF6

   Plasmid

   #44337

   Depositor

   Marc Timmers

   Article

   Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

   Insert

   TAF6
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mucin MUC1-5TR-pET15b

   Plasmid

   #174694

   Purpose

   Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

   Depositor

   Jean Gariepy

   Article

   Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

   Insert

   MUC1-TR
   

   Tags

   hexahistidine (HIS) tag

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 Promoter

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. ND4.2-Left TALE-G1397-N-DddA6

   Plasmid

   #179680

   Purpose

   Expression of base editor in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (Q1310R S1330I T1380I)_1xUGI_ATP5B 3'UTR
   

   Expression

   Mammalian

   Mutation

   Q1310R, S1330I, T1380I

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMuLE_ENTR_CBF-GLuc_L3-L2

    Plasmid

    #113868

    Purpose

    Single pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    CBF (notch dependent promoter)
    GLuc
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFSW GAP43-jRGECO1a P2A Syp-Halo

    Plasmid

    #185565

    Purpose

    Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

    Insert

    GAP43-jRGECO1a P2A Syp-Halo
    

    Use

    Lentiviral

    Tags

    HaloTag

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. opto-Itsn1

    Plasmid

    #174509

    Purpose

    Expresses opto-Itsn1 (Itsn1DH-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    opto-Itsn1-mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

    Plasmid

    #113864

    Purpose

    Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    PTCH1 (hedgehog dependent promoter)
    mTurquoise2
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. MBD-IFP2.0C

    Plasmid

    #215748

    Purpose

    Expresses MBD1 MBD domain fused to C-terminal part of IFP2.0 for 5mC detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    IFP2.0C
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. MBD_R44Q-IFP2.0C

    Plasmid

    #215749

    Purpose

    Expresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    IFP2.0C
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 2xHP1bCD-IFP2.0C

    Plasmid

    #215750

    Purpose

    Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2xHP1bCD-IFP2.0C
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. S1-Fc

    Plasmid

    #164220

    Purpose

    Expresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    Spike S1 domain (S SARS-CoV)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCK052 p5E exorh:mCherry

    Plasmid

    #195953

    Purpose

    Gateway p5E vector with reporter

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh:mCherry
    

    Use

    Other

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. G1397 DddAtox-N–dSpCas9

    Plasmid

    #157835

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
    

    Use

    CRISPR

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAP03-ermE*p

    Plasmid

    #120234

    Purpose

    Carries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    promoter cassette containing ermE*p promoter and ampicillin resistance gene
    

    Use

    Synthetic Biology

    Mutation

    bla (amp resistance gene)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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