1. Myr-(iSH2-p85)-p110alpha-Myc

   Plasmid

   #1410

   Depositor

   Lewis Cantley

   Insert

   PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pCMV-eNme2-C.NR

   Plasmid

   #185672

   Purpose

   Expresses nuclease active eNme2-C.NR in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   eNme2-C.NR nuclease
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…

   Promoter

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSLQ1651-sgRNA(F+E)-sgGal4

   Plasmid

   #100549

   Purpose

   sgGal4-expressing vector modified from Addgene plasmid #51024

   Depositor

   Jian Xu

   Article

   Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

   Insert

   sgGal4
   

   Use

   CRISPR

   Tags

   sgRNA

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDisplay-UDP1.0-IRES-mCherry-CAAX

   Plasmid

   #220850

   Purpose

   CMV promoter drives the expression of an extracellular UDP sensor (UDP1.0) and a membrane localized mCherry

   Depositor

   Yulong Li

   Article

   Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

   Insert

   UDP1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p240 pPr-CREM/Ins

   Plasmid

   #8405

   Purpose

   Androgen-inducible expression of Cre, with chimeric/β-globin intron within the Cre coding sequence and chicken globin insulators.

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   probasin driven CREM with insulators
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   modified Cre

   Promoter

   Probasin promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

5. p224 pCMV-RFP/CREM

   Plasmid

   #8401

   Purpose

   Floxed RFP inserted within the coding sequence of cre recombinase.

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   RFP/CREM
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   modified Cre, LOX 511 sites surrounding RFP withi…

   Promoter

   CMV

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

6. p236 pPr-CREM

   Plasmid

   #8404

   Purpose

   Expression of Cre from tissue-specific, androgen-inducible probasin promoter, with chimeric/β-globin intron within the Cre coding sequence.

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   probasin driven CREM
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   modified Cre

   Promoter

   Probasin promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

7. FAS promoter luciferase

   Plasmid

   #8890

   Depositor

   Bruce Spiegelman

   Article

   Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

   Insert

   Fatty Acid Synthetase Promoter (Fasn unknown - rat or mouse, Rat)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CAG-LiLac

   Plasmid

   #184570

   Purpose

   Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV

   Depositor

   Gary Yellen

   Article

   Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

   Insert

   LiLac fluorescent sensor of lactate
   

   Use

   AAV

   Tags

   His7

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(-)-LZ-CD95L

   Plasmid

   #104349

   Purpose

   Expresses human CD95L leucine zipper in mammalian cells

   Depositor

   Alexander McLellan

   Article

   Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

   Insert

   CD95L leucine zipper (FASLG Human)
   

   Use

   Tags

   octahistidine tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTPH413

    Plasmid

    #185728

    Purpose

    Bacterial PAM profiling of adenine base editor variants

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Inserts

    ecTadA(8e)-neNme2-C
    Nme2Cas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMXs-hFOXH1

    Plasmid

    #59183

    Purpose

    Forced expression of human FOXH1

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    FOXH1 (FOXH1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p255 pPr-CREM/Ins/CMV-STOP-luc

    Plasmid

    #8408

    Purpose

    Modified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM/insulators/lox/luciferase
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    Probasin, CMV

    Available since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pSUPERpuro-BglII-Hs4E-T_AC

    Plasmid

    #148445

    Purpose

    Mammalian Expression of Hs4E-T-shRNA

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    Hs4E-T-shRNA (EIF4ENIF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p252 pPr-CREM/CMV-STOP-luc

    Plasmid

    #8406

    Purpose

    Expression of modified Cre from androgen-inducible probasin promoter and LOX-dependent conditional reporter cassette.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM/lox/luciferase
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    Probasin promoter, CMV

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-hSyn-UDP1.0-WPRE

    Plasmid

    #220849

    Purpose

    AAV virus packaging plasmid for neuronal expression of an extracellular UDP sensor (UDP1.0)

    Depositor

    Yulong Li

    Article

    Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

    Insert

    UDP1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin (hSyn)

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-eNme2-C-ABE8e

    Plasmid

    #185667

    Purpose

    Expresses eNme2-C-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-C
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEF1a-FB-dCas9-puro

    Plasmid

    #100547

    Purpose

    Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Inserts

    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
    puromycin
    

    Use

    CRISPR

    Tags

    FLAG, Biotin-acceptor-site (Avitag)

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT7-EGFP-C1-Hs4E-T_AF

    Plasmid

    #148687

    Purpose

    Mammalian Expression of Hs4E-T

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    Hs4E-T (EIF4ENIF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-eNme2-C-BE4max

    Plasmid

    #183679

    Purpose

    Expresses eNme2-C-BE4 in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    rAPOBEC1-neNme2-C-2xUGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. DPYD-FLAG

    Plasmid

    #61616

    Purpose

    The human DPYD was used to study the effect of DPYD expression on EMT

    Depositor

    David Sabatini

    Article

    Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

    Insert

    Dihydropyrimidine dehydrogenase (DPYD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge