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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,700 results for: golden gate

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Showing: 3881 - 3900 of 4700 results

  4. SMT503 - S77A

    Plasmid
    #226842
    Purpose
    Plasmid that encodes a B. Subtilis lipase (CLIP-BsLipA-eGFP) gene fusion with the S77A catalytic mutant. Compatible with PURExpress for in vitro transcription/translation. Chloramp
    Depositor
    Polly Fordyce
    Article
    Thompson et al Adv Mater Interfaces. 2025 Feb 3;12(3):2
    Insert
    Lipase A
    Use
    Synthetic Biology
    Tags
    SNAP and eGFP
    Expression
    Bacterial
    Mutation
    R33Q, D34N, K35D, S77A, K112D, M134D, Y139C, I157M
    Promoter
    T7
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    226842-plasmid-map-sequence-id-456036

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  5. SMT503

    Plasmid
    #226840
    Purpose
    Plasmid that encodes a B. Subtilis lipase (BsLipA) gene fusion with N-terminal SNAP and C-Terminal eGFP under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. Chloramp
    Depositor
    Polly Fordyce
    Article
    Thompson et al Adv Mater Interfaces. 2025 Feb 3;12(3):2
    Insert
    Lipase A
    Use
    Synthetic Biology
    Tags
    SNAP and eGFP
    Expression
    Bacterial
    Mutation
    R33Q, D34N, K35D, K112D, M134D, Y139C, I157M
    Promoter
    T7
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    226840-plasmid-map-sequence-id-456035

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  7. pMKR07

    Plasmid
    #240097
    Purpose
    A CRISPR‑Cas9 system for knock‑out and knock‑in of high molecular weight DNA enables module‑swapping of the pikromycin synthase in its native host
    Depositor
    Adrian Keatinge-Clay
    Article
    Wang et al Res Sq [Preprint]. 2025 Mar 27:rs.3.rs-6
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    ermE* promoter
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    240097-plasmid-map-sequence-id-478442

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Showing: 3881 - 3900 of 4700 results