We narrowed to 15,158 results for: pET

1. pET mCerulean LIC cloning vector (H6-mCerulean)

   Plasmid

   #29726

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, AviTag (biotin), TEV cleavage site, and mC…

   Expression

   Bacterial

   Available Since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

   Plasmid

   #156463

   Purpose

   Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

   Depositor

   Andre Stiel

   Article

   Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

   Inserts

   Heme Oxygenase
   ReBphP-PCM
   

   Tags

   His Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-Duet1-His-TEV-mCherry-OPTN WT

   Plasmid

   #190191

   Purpose

   Plasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797

   Depositor

   Sascha Martens

   Article

   Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   optineurin (OPTN Human)
   

   Tags

   6xHis, TEV, and mCherry

   Expression

   Bacterial

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28-Strep-OPA1(253-960) (SB263)

   Plasmid

   #227612

   Purpose

   Inducible expression of Strep-tagged Opa1(253-960) in E.coli

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   OPA1 (OPA1 Human)
   

   Tags

   Twin-StrepTag

   Expression

   Bacterial

   Mutation

   contains aa 253-960 only

   Promoter

   T7

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB

   Plasmid

   #176587

   Purpose

   Bacterial expression of His14-MBP-TEV-AmaTnpB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Inserts

   MBP tag
   TnpB
   

   Tags

   His14 tag

   Expression

   Bacterial

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Affibody HER2:243.BirA*-pET301/NT-DEST

   Plasmid

   #174692

   Purpose

   Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*

   Depositor

   Jean Gariepy

   Article

   Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

   Insert

   Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
   

   Tags

   HIS tag

   Expression

   Bacterial

   Mutation

   R118G mutation in BirA

   Promoter

   T7 promoter

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)

   Plasmid

   #182395

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-LZ-VenusN154
   VNp-LZ-VenusC155
   

   Tags

   Vesicle Nucleating peptide (VNp) & Leucine Zi…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET tdTomato LIC cloning vector (u-tdTomato)

   Plasmid

   #86926

   Purpose

   pET LIC cloning vector for yORF-tdTomato

   Depositor

   Chris Jeans

   Type

   Empty backbone

   Tags

   tdTomato

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET mCitrine LIC cloning vector (u-mCitrine)

   Plasmid

   #29771

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   mCitrine

   Expression

   Bacterial

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET mCerulean LIC cloning vector (u-mCerulean)

    Plasmid

    #29773

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    mCerulean

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET PPL His6 LIC cloning vector (2J-T)

    Plasmid

    #37182

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and PPL

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. His-TEV-Aurein1.2_(+36GFP)-LPETG-His

    Plasmid

    #71755

    Purpose

    His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    Aurein1.2_(pos36_GFP)-LPETG-His
    

    Tags

    6XHis, Sortase (LPETG), and TEV Protease

    Expression

    Bacterial

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

    Plasmid

    #171587

    Purpose

    Bacterial expression of NLS-RfxCas13d-NLS 6His

    Depositor

    Neville Sanjana

    Article

    Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

    Insert

    MKIEE-NLS-RfxCas13d-NLS-HA-6His
    

    Tags

    HA-6HIS and MKIEE

    Expression

    Bacterial

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid

    #177335

    Purpose

    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase

    Depositor

    Christopher Overall

    Article

    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

    Insert

    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Tags

    Factor Xa site-3xFlag-Myc-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    Nov. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET28-His6-SUMO-cSrc-SH2-AviTag

    Plasmid

    #214230

    Purpose

    Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75

    Insert

    cSrc kinase SH2 domain (SRC Human)
    

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-GGG-HIS-CBM-Xmod-DocIII

    Plasmid

    #91698

    Purpose

    CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-HIS-CBM-Xmod-DocIII
    

    Tags

    HIS-Tag and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Cysteine deletion in CBM C63S

    Promoter

    T7

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. His6-Twin-Strep-SUMO-pET28a-EfaCas2

    Plasmid

    #112794

    Purpose

    To express enterococcus faecalis Cas2 protein in E. coli

    Depositor

    Ailong Ke

    Article

    Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

    Insert

    EfaCas2
    

    Tags

    His6-Twin-Strep-SUMO

    Expression

    Bacterial

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21b-Caspase-3(D3A: D175A D28A D9A)

    Plasmid

    #90090

    Purpose

    Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

    Depositor

    Clay Clark

    Article

    Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET StrepII TEV LIC cloning vector (2R-T)

    Plasmid

    #29717

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    StrepII-TEV

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

    Plasmid

    #217767

    Purpose

    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Protein purification (6xhis tag, tev site, sumo3 …

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    CA3 domain aa 317-400

    Promoter

    T7 promoter

    Available Since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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