We narrowed to 7,663 results for: ski

1. pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

   Plasmid

   #216244

   Purpose

   Express AtAFB2.F74A mutant with mCherry tag for AID2

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   AtAFB2.F74A-mCherry
   

   Use

   CRISPR, TALEN, and Unspecified ; Human safe harbo…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   F74A mutation

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQCXIZ GFP PODXL B WT

   Plasmid

   #202414

   Purpose

   Expression of GFP-tagged PODXL (isoform B) WT

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-puro-N-3HA-SREBP1-C-Flag

   Plasmid

   #134286

   Purpose

   Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SREBP1 (Srebf1 Mouse)
   

   Use

   Lentiviral

   Tags

   3x HA and Flag

   Expression

   Mammalian

   Mutation

   mouse SREBP1 isoform a precursor

   Promoter

   EF1a

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-C2m2-mKate

   Plasmid

   #208793

   Purpose

   For AAV production to express C2m2-mKate in astrocytes

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-mKate (Mfge8 Mouse)
   

   Use

   AAV

   Tags

   mKate

   Expression

   Mammalian

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   GFAP promoter

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-PEmax SpG

   Plasmid

   #187895

   Purpose

   All-in-one prime editor piggyBac transposon with PEmax, SpG variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   BPSV40 NLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX Grb2 SH2-SH3

   Plasmid

   #46442

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Grb2 (GRB2 Human)
   

   Tags

   GST and Thrombin site

   Expression

   Bacterial

   Mutation

   contains amino acids 1-217

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV2-CAG-3xNLS-vsfGFP-0

   Plasmid

   #191097

   Purpose

   To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-vsfGFP-0
   

   Use

   AAV

   Tags

   Green fluorescent protein bound to enhancer nanob…

   Expression

   Mammalian

   Mutation

   Optimized to Human codon usage

   Promoter

   CMV and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDY0750 pCAG-NLS-BceINT

   Plasmid

   #179112

   Purpose

   BceINT expression vector

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCAG-NLS-BceINT
   

   Use

   CRISPR

   Available Since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5 FRT/TO SF-HERC2 (ShB-R)

   Plasmid

   #55613

   Purpose

   Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (WT) (HERC2 Human)
   

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Silent mutations have been incorporated into the …

   Available Since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHLS-EF1a-FKBP12-Gag(HIV)

    Plasmid

    #138476

    Purpose

    Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    HIV Gag
    

    Tags

    FRBP12 and Lynn Myristolation site

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. W118-1_hTGM1-Flag

    Plasmid

    #180406

    Purpose

    lentiviral transduction of human TGM1 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM1 (TGM1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AcGFP-MSH2

    Plasmid

    #215121

    Purpose

    Expresses AcGFP-MSH2 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    MSH2 (MSH2 Human)
    

    Use

    Retroviral

    Tags

    AcGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CamKII-LGI1-pHluorin

    Plasmid

    #185537

    Purpose

    Expresses LGI1-pHluorin under the CamKII promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    LGI1 (Lgi1 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. CamKII-LGI1-pHmScarlet

    Plasmid

    #185538

    Purpose

    Expresses LGI1-pHmScarlet under the CamKII promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    LGI1 (Lgi1 Rat)
    

    Use

    Lentiviral

    Tags

    pHmScarlet

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR v2 sgNRF2-4

    Plasmid

    #186839

    Purpose

    knock out NRF2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

    Insert

    Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV HA hRb delta CDK

    Plasmid

    #58906

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. PL-5LTR-RGR(DMD​#​1​)-AmCyan-A

    Plasmid

    #138482

    Purpose

    Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Ribozyme-flanked gRNA and AmCyan
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-puro-N-3HA-SREBP2-C-Flag

    Plasmid

    #134287

    Purpose

    Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP2 (Srebf2 Mouse)
    

    Use

    Lentiviral

    Tags

    3x HA and Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP2 precursor

    Promoter

    CMV

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lentiCRISPR v2 sgNRF2-3

    Plasmid

    #186838

    Purpose

    knock out NRF2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

    Insert

    Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX SHIP1-SH2

    Plasmid

    #46492

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHIP1 (INPP5D Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-118

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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